ExplorEnz: EC 2.5.1.47

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2.5.1.47

Accepted name:

cysteine synthase

Reaction:

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

For diagram of O³-Acetyl-L-serine metabolism, click here

Glossary:

O-acetyl-L-serine = (2S)-3-acetyloxy-2-aminopropanoic acid

Other name(s):

O-acetyl-L-serine sulfhydrylase; O-acetyl-L-serine sulfohydrolase; O-acetylserine (thiol)-lyase; O-acetylserine (thiol)-lyase A; O-acetylserine sulfhydrylase; O³-acetyl-L-serine acetate-lyase (adding hydrogen-sulfide); acetylserine sulfhydrylase; cysteine synthetase; S-sulfocysteine synthase; 3-O-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase; O³-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

Systematic name:

O-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

Comments:

A pyridoxal-phosphate protein. Some alkyl thiols, cyanide, pyrazole and some other heterocyclic compounds can act as acceptors. Not identical with EC 2.5.1.51 (β-pyrazolylalanine synthase), EC 2.5.1.52 (L-mimosine synthase) and EC 2.5.1.53 (uracilylalanine synthase).

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 37290-89-4

References:

1.	Becker, M.A., Kredich, N.M. and Tomkins, G.M. The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium. J. Biol. Chem. 244 (1969) 2418–2427. [PMID: 4891157]
2.	Hara, S., Payne, M.A., Schnackerz, K.D. and Cook, P.F. A rapid purification procedure and computer-assisted sulfide ion selective electrode assay for O-acetylserine sulfhydrylase from Salmonella typhimurium. Protein Expr. Purif. 1 (1990) 70–76. [PMID: 2152186]
3.	Ikegami, F., Kaneko, M., Lambein, F., Kuo, Y.-H. and Murakoshi, I. Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum. Phytochemistry 26 (1987) 2699–2704.
4.	Murakoshi, I., Kaneko, M., Koide, C. and Ikegami, F. Enzymatic-synthesis of the neuroexcitatory amino-acid quisqualic by cysteine synthase. Phytochemistry 25 (1986) 2759–2763.
5.	Tai, C.H., Burkhard, P., Gani, D., Jenn, T., Johnson, C. and Cook, P.F. Characterization of the allosteric anion-binding site of O-acetylserine sulfhydrylase. Biochemistry 40 (2001) 7446–7452. [DOI] [PMID: 11412097]
6.	Bettati, S., Benci, S., Campanini, B., Raboni, S., Chirico, G., Beretta, S., Schnackerz, K.D., Hazlett, T.L., Gratton, E. and Mozzarelli, A. Role of pyridoxal 5′-phosphate in the structural stabilization of O-acetylserine sulfhydrylase. J. Biol. Chem. 275 (2000) 40244–40251. [DOI] [PMID: 10995767]

[EC 2.5.1.47 created 1972 as EC 4.2.99.8, modified 1976, modified 1990, transferred 2002 to EC 2.5.1.47]