EC |
2.6.1.16 |
Accepted name: |
glutamine—fructose-6-phosphate transaminase (isomerizing) |
Reaction: |
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate |
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For diagram of the biosynthesis of UDP-N-acetylglucosamine, click here |
Other name(s): |
hexosephosphate aminotransferase; glucosamine-6-phosphate isomerase (glutamine-forming); glutamine-fructose-6-phosphate transaminase (isomerizing); D-fructose-6-phosphate amidotransferase; glucosaminephosphate isomerase; glucosamine 6-phosphate synthase; GlcN6P synthase |
Systematic name: |
L-glutamine:D-fructose-6-phosphate isomerase (deaminating) |
Comments: |
Although the overall reaction is that of a transferase, the mechanism involves the formation of ketimine between fructose 6-phosphate and a 6-amino group from a lysine residue at the active site, which is subsequently displaced by ammonia (transamidination). |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9030-45-9 |
References: |
1. |
Ghosh, S., Blumenthal, H.J., Davidson, E. and Roseman, S. Glucosamine metabolism. V. Enzymatic synthesis of glucosamine 6-phosphate. J. Biol. Chem. 235 (1960) 1265–1273. [PMID: 13827775] |
2. |
Gryder, R.M. and Pogell, B.M. Further studies on glucosamine 6-phosphate synthesis by rat liver enzymes. J. Biol. Chem. 235 (1960) 558–562. [PMID: 13829889] |
3. |
Leloir, L.F. and Cardini, C.E. The biosynthesis of glucosamine. Biochim. Biophys. Acta 12 (1953) 15–22. [DOI] [PMID: 13115409] |
4. |
Teplyakov, A., Obmolova, G., Badet-Denisot, M.A. and Badet, B. The mechanism of sugar phosphate isomerization by glucosamine 6-phosphate synthase. Protein Sci. 8 (1999) 596–602. [DOI] [PMID: 10091662] |
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[EC 2.6.1.16 created 1961, deleted 1972, reinstated 1984, modified 2000 (EC 5.3.1.19 created 1972, incorporated 1984)] |
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