| EC |
2.7.7.6 |
| Accepted name: |
DNA-directed RNA polymerase |
| Reaction: |
nucleoside triphosphate + RNAn = diphosphate + RNAn+1 |
| Other name(s): |
RNA polymerase; RNA nucleotidyltransferase (DNA-directed); RNA polymerase I; RNA polymerase II; RNA polymerase III; C RNA formation factors; deoxyribonucleic acid-dependent ribonucleic acid polymerase; DNA-dependent ribonucleate nucleotidyltransferase; DNA-dependent RNA nucleotidyltransferase; DNA-dependent RNA polymerase; ribonucleate nucleotidyltransferase; ribonucleate polymerase; C ribonucleic acid formation factors; ribonucleic acid nucleotidyltransferase; ribonucleic acid polymerase; ribonucleic acid transcriptase; ribonucleic polymerase; ribonucleic transcriptase; RNA nucleotidyltransferase; RNA transcriptase; transcriptase; RNA nucleotidyltransferase I |
| Systematic name: |
nucleoside-triphosphate:RNA nucleotidyltransferase (DNA-directed) |
| Comments: |
Catalyses DNA-template-directed extension of the 3′- end of an RNA strand by one nucleotide at a time. Can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to α-amanitin, and the type of RNA synthesized. See also EC 2.7.7.19 (polynucleotide adenylyltransferase) and EC 2.7.7.48 (RNA-directed RNA polymerase). |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9014-24-8 |
| References: |
| 1. |
Krakow, J.S. and Ochoa, S. RNA polymerase from Azotobacter vinelandii. Methods Enzymol. 6 (1963) 11–17. |
| 2. |
Mans, R.J. and Walter, T.J. Transfer RNA-primed oligoadenylate synthesis in maize seedlings. II. Primer, substrate and metal specificities and size of product. Biochim. Biophys. Acta 247 (1971) 113–121. [DOI] [PMID: 4946277] |
| 3. |
Roeder, R.G. In: Losick, R. and Chamberlin, M. (Ed.), RNA Polymerase, Cold Spring Harbor Laboratory, 1976, p. 285. |
| 4. |
Sheldon, R., Jurale, C. and Kates, J. Detection of polyadenylic acid sequences in viral and eukaryotic RNA(poly(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4). Proc. Natl. Acad. Sci. USA 69 (1972) 417–421. [DOI] [PMID: 4501121] |
| 5. |
Weaver, R.F., Blatti, S.P. and Rutter, W.J. Molecular structures of DNA-dependent RNA polymerases (II) from calf thymus and rat liver. Proc. Natl. Acad. Sci. USA 68 (1971) 2994–2999. [DOI] [PMID: 5289245] |
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| [EC 2.7.7.6 created 1961, modified 1981, modified 1982, modified 1989] |
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