ExplorEnz: EC 2.7.7.7

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2.7.7.7

Accepted name:

DNA-directed DNA polymerase

Reaction:

a 2′-deoxyribonucleoside 5′-triphosphate + DNA_n = diphosphate + DNA_n+1

Other name(s):

DNA polymerase I; DNA polymerase II; DNA polymerase III; DNA polymerase α; DNA polymerase β; DNA polymerase γ; DNA nucleotidyltransferase (DNA-directed); deoxyribonucleate nucleotidyltransferase; deoxynucleate polymerase; deoxyribonucleic acid duplicase; deoxyribonucleic acid polymerase; deoxyribonucleic duplicase; deoxyribonucleic polymerase; deoxyribonucleic polymerase I; DNA duplicase; DNA nucleotidyltransferase; DNA polymerase; DNA replicase; DNA-dependent DNA polymerase; duplicase; Klenow fragment; sequenase; Taq DNA polymerase; Taq Pol I; Tca DNA polymerase

Systematic name:

2′-deoxyribonucleoside-5′-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed)

Comments:

Catalyses DNA-template-directed extension of the 3′- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. Requires a primer, which may be DNA or RNA. See also EC 2.7.7.49 RNA-directed DNA polymerase.

Links to other databases:

BRENDA, EXPASY, GTD, KEGG, MetaCyc, PDB, CAS registry number: 9012-90-2

References:

1.	Bollum, F.J. Calf thymus polymerase. J. Biol. Chem. 235 (1960) 2399–2403. [PMID: 13802334]
2.	Falaschi, A. and Kornberg, A. Biochemical studies of bacterial sporulation. II. Deoxy-ribonucleic acid polymerase in spores of Bacillus subtilis. J. Biol. Chem. 241 (1966) 1478–1482. [PMID: 4957767]
3.	Lehman, I.R., Bessman, M.J., Simms, E.S. and Kornberg, A. Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J. Biol. Chem. 233 (1958) 163–170. [PMID: 13563462]
4.	Richardson, C.C., Schildkraut, C.L., Aposhian, H.V. and Kornberg, A. Enzymatic synthesis of deoxyribonucleic acid. XIV. Further purification and properties of deoxyribonucleic acid polymerase of Escherichia coli. J. Biol. Chem. 239 (1964) 222–232. [PMID: 14114848]
5.	Schachman, H.K., Adler, J., Radding, C.M., Lehman, I.R. and Kornberg, A. Enzymatic synthesis of deoxyribonucleic acid. VII. Synthesis of a polymer of deoxyadenylate and deoxythymidylate. J. Biol. Chem. 235 (1960) 3242–3249. [PMID: 13747134]
6.	Zimmerman, B.K. Purification and properties of deoxyribonucleic acid polymerase from Micrococcus lysodeikticus. J. Biol. Chem. 241 (1966) 2035–2041. [PMID: 5946628]

[EC 2.7.7.7 created 1961, modified 1981, modified 1982]