EC |
2.7.7.88 |
Accepted name: |
GDP polyribonucleotidyltransferase |
Reaction: |
(5′)pppAACA-[mRNA] + GDP = diphosphate + G(5′)pppAACA-[mRNA] (overall reaction) (1a) (5′)pppAACA-[mRNA] + [protein L]-L-histidine = diphosphate + [protein L]-L-histidyl-(5′)phosphonato-AACA-[mRNA] + H2O (1b) [protein L]-L-histidyl-(5′)phosphonato-AACA-[mRNA] + GDP + H2O = [protein L]-L-histidine + G(5′)pppAACA-[mRNA] |
Other name(s): |
PRNTase; 5′-triphospho-mRNA:GDP 5′-phosphopolyribonucleotidyltransferase [G(5′)ppp-mRNA-forming] |
Systematic name: |
(5′)pppAACA-[mRNA]:GDP 5′-phosphopolyribonucleotidyltransferase [(5′)pppAACA-[mRNA]-forming] |
Comments: |
The enzyme from non-segmented negative strain (NNS) viruses (e.g. rhabdoviruses and lyssaviruses) is specific for mRNAs with sequences starting with AACA. cf. EC 2.7.7.50, mRNA guanylyltransferase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Ogino, T. and Banerjee, A.K. Unconventional mechanism of mRNA capping by the RNA-dependent RNA polymerase of vesicular stomatitis virus. Mol. Cell 25 (2007) 85–97. [DOI] [PMID: 17218273] |
2. |
Ogino, T. and Banerjee, A.K. Formation of guanosine(5′)tetraphospho(5′)adenosine cap structure by an unconventional mRNA capping enzyme of vesicular stomatitis virus. J. Virol. 82 (2008) 7729–7734. [DOI] [PMID: 18495767] |
3. |
Ogino, T., Yadav, S.P. and Banerjee, A.K. Histidine-mediated RNA transfer to GDP for unique mRNA capping by vesicular stomatitis virus RNA polymerase. Proc. Natl. Acad. Sci. USA 107 (2010) 3463–3468. [DOI] [PMID: 20142503] |
4. |
Ogino, T. and Banerjee, A.K. The HR motif in the RNA-dependent RNA polymerase L protein of Chandipura virus is required for unconventional mRNA-capping activity. J. Gen. Virol. 91 (2010) 1311–1314. [DOI] [PMID: 20107017] |
5. |
Ogino, T. and Banerjee, A.K. An unconventional pathway of mRNA cap formation by vesiculoviruses. Virus Res. 162 (2011) 100–109. [DOI] [PMID: 21945214] |
6. |
Ogino, M., Ito, N., Sugiyama, M. and Ogino, T. The rabies virus L protein catalyzes mRNA capping with GDP polyribonucleotidyltransferase activity. Viruses 8:144 (2016). [DOI] [PMID: 27213429] |
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[EC 2.7.7.88 created 2015, modified 2020] |
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