EC |
2.7.99.1 |
Accepted name: |
triphosphate—protein phosphotransferase |
Reaction: |
triphosphate + [microsomal-membrane protein] = diphosphate + phospho-[microsomal-membrane protein] |
Other name(s): |
diphosphate:microsomal-membrane-protein O-phosphotransferase (erroneous); DiPPT (erroneous); pyrophosphate:protein phosphotransferase (erroneous); diphosphate—protein phosphotransferase (erroneous); diphosphate:[microsomal-membrane-protein] O-phosphotransferase (erroneous) |
Systematic name: |
triphosphate:[microsomal-membrane-protein] phosphotransferase |
Comments: |
This enzyme was originally thought to use diphosphate as substrate [1] but this has since been disproved [2]. The activity is observed as the second part of a biphasic reaction after depletion of ATP. Tripolyphosphate is a contaminant of [γ-32P]ATP. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 74092-32-3 |
References: |
1. |
Lam, K.S. and Kasper, C.B. Pyrophosphate:protein phosphotransferase: a membrane-bound enzyme of endoplasmic reticulum. Proc. Natl. Acad. Sci. USA 77 (1980) 1927–1931. [DOI] [PMID: 6246514] |
2. |
Tsutsui, K. Tripolyphosphate is an alternative phosphodonor of the selective protein phosphorylation of liver microsomal membrane. J. Biol. Chem. 261 (1986) 2645–2653. [PMID: 3949741] |
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[EC 2.7.99.1 created 1983 as EC 2.7.1.104, transferred 2005 to EC 2.7.99.1] |
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