| EC |
2.8.1.13 |
| Accepted name: |
tRNA-uridine 2-sulfurtransferase |
| Reaction: |
a [protein]-S-sulfanyl-L-cysteine + uracil34 in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouracil34 in tRNA + AMP + diphosphate + acceptor |
| Other name(s): |
mnmA (gene name) |
| Systematic name: |
[protein]-S-sulfanyl-L-cysteine:tRNA (uracil34-2-O)-sulfurtransferase |
| Comments: |
The enzyme, found in bacteria, catalyses formation of the 2-thiouridine modification in the wobble position of tRNAGln, tRNALys and tRNAGlu. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
| References: |
| 1. |
Kambampati, R. and Lauhon, C.T. MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli. Biochemistry 42 (2003) 1109–1117. [DOI] [PMID: 12549933] |
| 2. |
Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A. and Suzuki, T. Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions. Mol. Cell 21 (2006) 97–108. [DOI] [PMID: 16387657] |
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| [EC 2.8.1.13 created 2015] |
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