ExplorEnz: EC 2.8.1.15

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2.8.1.15

Accepted name:

tRNA-5-methyluridine⁵⁴ 2-sulfurtransferase

Reaction:

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluracil⁵⁴ in tRNA + H₂O = AMP + diphosphate + 5-methyl-2-thiouracil⁵⁴ in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly

Other name(s):

TtuA

Systematic name:

[TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH:tRNA (5-methyluridine⁵⁴-2-O)-sulfurtransferase

Comments:

The enzyme, found in thermophilic bacteria and archaea, modifies the ribothymidine (5-methyluridine) residue at position 54 of tRNAs. Contains zinc and an [4Fe-4S] cluster. Some organisms, such as the archaeon Pyrococcus horikoshii, do not have a TtuB sulfur-carrier protein, and appear to use sulfide as the sulfur source.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Shigi, N., Sakaguchi, Y., Suzuki, T. and Watanabe, K. Identification of two tRNA thiolation genes required for cell growth at extremely high temperatures. J. Biol. Chem. 281 (2006) 14296–14306. [DOI] [PMID: 16547008]
2.	Shigi, N., Suzuki, T., Terada, T., Shirouzu, M., Yokoyama, S. and Watanabe, K. Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA. J. Biol. Chem. 281 (2006) 2104–2113. [DOI] [PMID: 16317006]
3.	Nakagawa, H., Kuratani, M., Goto-Ito, S., Ito, T., Katsura, K., Terada, T., Shirouzu, M., Sekine, S., Shigi, N. and Yokoyama, S. Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA. Proteins 81 (2013) 1232–1244. [DOI] [PMID: 23444054]
4.	Chen, M., Narai, S., Omura, N., Shigi, N., Chimnaronk, S., Tanaka, Y. and Yao, M. Crystallographic study of the 2-thioribothymidine-synthetic complex TtuA-TtuB from Thermus thermophilus. Acta Crystallogr. F Struct. Biol. Commun. 72 (2016) 777–781. [DOI] [PMID: 27710943]

[EC 2.8.1.15 created 2017]