ExplorEnz: EC 2.8.2.22

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2.8.2.22

Accepted name:

aryl-sulfate sulfotransferase

Reaction:

an aryl sulfate + a phenol = a phenol + an aryl sulfate

Other name(s):

arylsulfate-phenol sulfotransferase; arylsulfotransferase (ambiguous); ASST; arylsulfate sulfotransferase; arylsulfate:phenol sulfotransferase; astA (gene name); aryl-sulfate:phenol sulfotransferase

Systematic name:

aryl-sulfate:phenol sulfonotransferase

Comments:

The enzyme, characterized from bacteria that colonize the human and mouse intestine, catalyses the transfer of a sulfate group from a phenol sulfate ester to other phenolic compounds. Activity is enhanced by Mg²⁺ and Mn²⁺ [1]. Unlike EC 2.8.2.9, tyrosine-ester sulfotransferase and EC 2.8.2.1, aryl sulfotransferase, the enzyme does not act on 3′-phosphoadenylyl sulfate or adenosine 3′,5′-bisphosphate [1].The level of sulfation of polyphenols depends on the positions of the hydroxyl groups [3-5]. Hydroxy groups of tyrosine residues in peptides such as angiotensin can also act as acceptors [2]. The reaction proceeds according to a ping pong bi bi mechanism [6].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 158254-86-5

References:

1.	Kim, D.-H., Konishi, L. and Kobashi, K. Purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine. Biochim. Biophys. Acta 872 (1986) 33–41. [DOI] [PMID: 3460636]
2.	Kobashi, K., Kim, D.-H. and Morikawa, T. A novel type of arylsulfotransferase. J. Protein Chem. 6 (1987) 237–244.
3.	Koizumi, M., Shimizu, M. and Kobashi, K. Enzymatic sulfation of quercetin by arylsulfotransferase from a human intestinal bacterium. Chem Pharm Bull (Tokyo) 38 (1990) 794–796. [PMID: 2347024]
4.	Koizumi, M., Akao, T., Kadota, S., Kikuchi, T., Okuda, T. and Kobashi, K. Enzymatic sulfation of polyphenols related to tannins by arylsulfotransferase. Chem Pharm Bull (Tokyo) 39 (1991) 2638–2643. [PMID: 1806284]
5.	Konishi-Imamura, L., Sato, M., Dohi, K., Kadota, S., Namba, T. and Kobashi, K. Enzymatic sulfation of glycosides and their corresponding aglycones by arylsulfate sulfotransferase from a human intestinal bacterium. Biol. Pharm. Bull. 17 (1994) 1018–1022. [PMID: 7820100]
6.	Lee, N.S., Kim, B.T., Kim, D.H. and Kobashi, K. Purification and reaction mechanism of arylsulfate sulfotransferase from Haemophilus K-12, a mouse intestinal bacterium. J. Biochem. 118 (1995) 796–801. [PMID: 8576095]
7.	Kim, B., Hyun, Y.J., Lee, K.S., Kobashi, K. and Kim, D.H. Cloning, expression and purification of arylsulfate sulfotransferase from Eubacterium A-44. Biol. Pharm. Bull. 30 (2007) 11–14. [PMID: 17202651]

[EC 2.8.2.22 created 1990]