ExplorEnz: EC 2.8.5.2

Your query returned 1 entry. Printable version

2.8.5.2

Accepted name:

L-cysteine S-thiosulfotransferase

Reaction:

(1) [SoxY protein]-L-cysteine + thiosulfate + 2 ferricytochrome c = [SoxY protein]-S-sulfosulfanyl-L-cysteine + 2 ferrocytochrome c + 2 H⁺
(2) [SoxY protein]-S-sulfanyl-L-cysteine + thiosulfate + 2 ferricytochrome c = [SoxY protein]-S-(2-sulfodisulfanyl)-L-cysteine + 2 ferrocytochrome c + 2 H⁺

Other name(s):

SoxXA; thiosulfate:[SoxY protein]-L-cysteine thiosulfotransferase

Systematic name:

thiosulfate:[SoxY protein]-L-cysteine thiosulfonotransferase

Comments:

The enzyme is part of the Sox enzyme system, which participates in a bacterial thiosulfate oxidation pathway that produces sulfate. It catalyses two reactions in the pathway - early in the pathway it attaches a thiosulfate molecule to the sulfur atom of an L-cysteine of a SoxY protein; later it transfers a second thiosulfate molecule to a sulfane group that is already attached to the same cysteine residue.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Friedrich, C.G., Quentmeier, A., Bardischewsky, F., Rother, D., Kraft, R., Kostka, S. and Prinz, H. Novel genes coding for lithotrophic sulfur oxidation of Paracoccus pantotrophus GB17. J. Bacteriol. 182 (2000) 4677–4687. [PMID: 10940005]
2.	Cheesman, M.R., Little, P.J. and Berks, B.C. Novel heme ligation in a c-type cytochrome involved in thiosulfate oxidation: EPR and MCD of SoxAX from Rhodovulum sulfidophilum. Biochemistry 40 (2001) 10562–10569. [PMID: 11523998]
3.	Rother, D. and Friedrich, C.G. The cytochrome complex SoxXA of Paracoccus pantotrophus is produced in Escherichia coli and functional in the reconstituted sulfur-oxidizing enzyme system. Biochim. Biophys. Acta 1598 (2002) 65–73. [PMID: 12147345]
4.	Bamford, V.A., Bruno, S., Rasmussen, T., Appia-Ayme, C., Cheesman, M.R., Berks, B.C. and Hemmings, A.M. Structural basis for the oxidation of thiosulfate by a sulfur cycle enzyme. EMBO J. 21 (2002) 5599–5610. [PMID: 12411478]
5.	Dambe, T., Quentmeier, A., Rother, D., Friedrich, C. and Scheidig, A.J. Structure of the cytochrome complex SoxXA of Paracoccus pantotrophus, a heme enzyme initiating chemotrophic sulfur oxidation. J. Struct. Biol. 152 (2005) 229–234. [PMID: 16297640]
6.	Hensen, D., Sperling, D., Truper, H.G., Brune, D.C. and Dahl, C. Thiosulphate oxidation in the phototrophic sulphur bacterium Allochromatium vinosum. Mol. Microbiol. 62 (2006) 794–810. [PMID: 16995898]
7.	Grabarczyk, D.B. and Berks, B.C. Intermediates in the Sox sulfur oxidation pathway are bound to a sulfane conjugate of the carrier protein SoxYZ. PLoS One 12:e0173395 (2017). [DOI] [PMID: 28257465]

[EC 2.8.5.2 created 2018]