| EC |
3.1.1.78 |
| Accepted name: |
polyneuridine-aldehyde esterase |
| Reaction: |
polyneuridine aldehyde + H2O = 16-epivellosimine + CO2 + methanol |
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For diagram of geissoschizine and sarpagine biosynthesis, click here |
| Other name(s): |
polyneuridine aldehyde esterase; PNAE |
| Systematic name: |
polyneuridine aldehyde hydrolase (decarboxylating) |
| Comments: |
Following hydrolysis of this indole alkaloid ester the carboxylic acid decarboxylates spontaneously giving the sarpagan skeleton. The enzyme also acts on akuammidine aldehyde (the 16-epimer of polyneuridine aldehyde). |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 87041-55-2 |
| References: |
| 1. |
Pfitzner, A. and Stöckigt, J. Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine ajmaline type alkaloids. Planta Med. 48 (1983) 221–227. [PMID: 17404987] |
| 2. |
Pfitzner, A. and Stöckigt, J. Polyneuridine aldehyde esterase: an unusual specific enzyme involved in the biosynthesis of sarpagine type alkaloids. J. Chem. Soc. Chem. Commun. (1983) 459–460. |
| 3. |
Dogru, E., Warzecha, H., Seibel, F., Haebel, S., Lottspeich, F. and Stöckigt, J. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the hydrolase super family. Eur. J. Biochem. 267 (2000) 1397–1406. [DOI] [PMID: 10691977] |
| 4. |
Mattern-Dogru, E., Ma, X., Hartmann, J., Decker, H. and Stöckigt, J. Potential active-site residues in polyneuridine aldehyde esterase, a
central enzyme of indole alkaloid biosynthesis, by modelling and
site-directed mutagenesis. Eur. J. Biochem. 269 (2002) 2889–2896. [DOI] [PMID: 12071952] |
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| [EC 3.1.1.78 created 2002] |
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