EC 3.1.1.78     
Accepted name: polyneuridine-aldehyde esterase
Reaction: polyneuridine aldehyde + H2O = 16-epivellosimine + CO2 + methanol
Other name(s): polyneuridine aldehyde esterase; PNAE
Systematic name: polyneuridine aldehyde hydrolase (decarboxylating)
Comments: Following hydrolysis of this indole alkaloid ester the carboxylic acid decarboxylates spontaneously giving the sarpagan skeleton. The enzyme also acts on akuammidine aldehyde (the 16-epimer of polyneuridine aldehyde).
References:
1.  Pfitzner, A. and Stöckigt, J. Characterization of polyneuridine aldehyde esterase, a key enzyme in the biosynthesis of sarpagine ajmaline type alkaloids. Planta Med. 48 (1983) 221–227. [PMID: 17404987]
2.  Pfitzner, A. and Stöckigt, J. Polyneuridine aldehyde esterase: an unusual specific enzyme involved in the biosynthesis of sarpagine type alkaloids. J. Chem. Soc. Chem. Commun. (1983) 459–460.
3.  Dogru, E., Warzecha, H., Seibel, F., Haebel, S., Lottspeich, F. and Stöckigt, J. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the hydrolase super family. Eur. J. Biochem. 267 (2000) 1397–1406. [PMID: 10691977]
4.  Mattern-Dogru, E., Ma, X., Hartmann, J., Decker, H. and Stöckigt, J. Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis. Eur. J. Biochem. 269 (2002) 2889–2896. [PMID: 12071952]
[EC 3.1.1.78 created 2002]