ExplorEnz: EC 3.1.1.96

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3.1.1.96

Accepted name:

D-aminoacyl-tRNA deacylase

Reaction:

(1) a D-aminoacyl-tRNA + H₂O = a D-amino acid + tRNA
(2) glycyl-tRNA^Ala + H₂O = glycine + tRNA^Ala

Other name(s):

Dtd2; D-Tyr-tRNA(Tyr) deacylase; D-Tyr-tRNA^Tyr deacylase; D-tyrosyl-tRNA^Tyr aminoacylhydrolase; dtdA (gene name)

Systematic name:

D-aminoacyl-tRNA aminoacylhydrolase

Comments:

The enzyme, found in all domains of life, can cleave mischarged glycyl-tRNA^Ala [5]. The enzyme from Escherichia coli can cleave D-tyrosyl-tRNA^Tyr, D-aspartyl-tRNA^Asp and D-tryptophanyl-tRNA^Trp [1]. Whereas the enzyme from the archaeon Pyrococcus abyssi is a zinc protein, the enzyme from Escherichia coli does not carry any zinc [2].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Soutourina, J., Plateau, P. and Blanquet, S. Metabolism of D-aminoacyl-tRNAs in Escherichia coli and Saccharomyces cerevisiae cells. J. Biol. Chem. 275 (2000) 32535–32542. [DOI] [PMID: 10918062]
2.	Ferri-Fioni, M.L., Schmitt, E., Soutourina, J., Plateau, P., Mechulam, Y. and Blanquet, S. Structure of crystalline D-Tyr-tRNA(Tyr) deacylase. A representative of a new class of tRNA-dependent hydrolases. J. Biol. Chem. 276 (2001) 47285–47290. [DOI] [PMID: 11568181]
3.	Ferri-Fioni, M.L., Fromant, M., Bouin, A.P., Aubard, C., Lazennec, C., Plateau, P. and Blanquet, S. Identification in archaea of a novel D-Tyr-tRNA^Tyr deacylase. J. Biol. Chem. 281 (2006) 27575–27585. [DOI] [PMID: 16844682]
4.	Wydau, S., Ferri-Fioni, M.L., Blanquet, S. and Plateau, P. GEK1, a gene product of Arabidopsis thaliana involved in ethanol tolerance, is a D-aminoacyl-tRNA deacylase. Nucleic Acids Res. 35 (2007) 930–938. [DOI] [PMID: 17251192]
5.	Pawar, K.I., Suma, K., Seenivasan, A., Kuncha, S.K., Routh, S.B., Kruparani, S.P. and Sankaranarayanan, R. Role of D-aminoacyl-tRNA deacylase beyond chiral proofreading as a cellular defense against glycine mischarging by AlaRS. Elife 6:e24001 (2017). [DOI] [PMID: 28362257]

[EC 3.1.1.96 created 2014, modified 2019]