| EC |
3.1.21.5 |
| Accepted name: |
type III site-specific deoxyribonuclease |
| Reaction: |
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5′-phosphates |
| Other name(s): |
type III restriction enzyme; restriction-modification system |
| Comments: |
This is a large group of enzymes which, together with those now listed as EC 3.1.21.3 (type I site-specific deoxyribonuclease) and EC 3.1.21.4 (type II site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP but do not hydrolyse it; S-adenosy-L-methionine stimulates the reaction, but is not absolutely required. They recognize specific, short DNA sequences and cleave a short distance away from the recognition sequence. These enzymes exist as complexes with enzymes of similar specificity listed under EC 2.1.1.72 [site-specific DNA-methyltransferase (adenine-specific)] or EC 2.1.1.37 [DNA (cytosine-5-)-methyltransferase]. |
| Links to other databases: |
BRENDA, EXPASY, GENE, KEGG, MetaCyc, PDB, CAS registry number: 9075-08-5 |
| References: |
| 1. |
Roberts, R.J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 18 (1990) 2331–2365. [PMID: 2159140] |
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| [EC 3.1.21.5 created 1984 from EC 3.1.23 and EC 3.1.24] |
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