The Enzyme Database

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Accepted name: type III site-specific deoxyribonuclease
Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5′-phosphates
Other name(s): type III restriction enzyme; restriction-modification system
Comments: This is a large group of enzymes which, together with those now listed as EC (type 1 site-specific deoxyribonuclease) and EC (type II site-specific deoxyribonuclease), were previously listed separately in sub-subclasses EC 3.1.23 and EC 3.1.24. They have an absolute requirement for ATP but do not hydrolyse it; S-adenosy-L-methionine stimulates the reaction, but is not absolutely required. They recognize specific, short DNA sequences and cleave a short distance away from the recognition sequence. These enzymes exist as complexes with enzymes of similar specificity listed under EC [site-specific DNA-methyltransferase (adenine-specific)] or EC
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9075-08-5
1.  Roberts, R.J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 18 (1990) 2331–2365. [PMID: 2159140]
[EC created 1984 from EC 3.1.23 and EC 3.1.24]

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