| EC |
3.2.1.156 |
| Accepted name: |
oligosaccharide reducing-end xylanase |
| Reaction: |
Hydrolysis of (1→4)-β-D-xylose residues from the reducing end of oligosaccharides |
| Other name(s): |
Rex; reducing end xylose-releasing exo-oligoxylanase |
| Systematic name: |
β-D-xylopyranosyl-(1→4)-β-D-xylopyranose reducing-end xylanase |
| Comments: |
The enzyme, originally isolated from the bacterium Bacillus halodurans C-125, releases the xylose unit at the reducing end of oligosaccharides ending with the structure β-D-xylopyranosyl-(1→4)-β-D-xylopyranosyl-(1→4)-β-D-xylopyranose, leaving the new reducing end in the α configuration. It is specific for the β anomers of xylooligosaccharides whose degree of polymerization is equal to or greater than 3.
The penultimate residue must be β-D-xylopyranose, but replacing either of the flanking residues with glucose merely slows the rate greatly. |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 879497-03-7 |
| References: |
| 1. |
Honda, Y. and Kitaoka, M. A family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase. J. Biol. Chem. 279 (2004) 55097–55103. [DOI] [PMID: 15491996] |
| 2. |
Fushinobu, S., Hidaka, M., Honda, Y., Wakagi, T., Shoun, H. and Kitaoka, M. Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125. J. Biol. Chem. 280 (2005) 17180–17186. [DOI] [PMID: 15718242] |
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| [EC 3.2.1.156 created 2005] |
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