ExplorEnz: EC 3.2.1.217

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3.2.1.217

Accepted name:

exo-acting protein-α-N-acetylgalactosaminidase

Reaction:

a [protein]-N-acetyl-α-D-galactosalaminyl-(L-serine/L-threonine) + H₂O = a [protein]-(L-serine/L-threonine) + N-acetyl-D-galactosamine

Other name(s):

Nag31

Systematic name:

[protein]-N-acetyl-α-D-galactosalaminyl-(L-serine/L-threonine) N-acetylgalactosaminohydrolase

Comments:

The enzyme, which belongs to the glycosylhydrolase 31 (GH31) family, is an exo-acting α-N-acetylgalactosaminidase that acts on the innermost α-GalNAc residues at the core of O-glycans when no other saccharides are attached to it. Unlike EC 3.2.1.49, α-N-acetylgalactosaminidase, it is not able to act on blood group A antigen.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Rahfeld, P., Wardman, J.F., Mehr, K., Huff, D., Morgan-Lang, C., Chen, H.M., Hallam, S.J. and Withers, S.G. Prospecting for microbial α-N-acetylgalactosaminidases yields a new class of GH31 O-glycanase. J. Biol. Chem. 294 (2019) 16400–16415. [DOI] [PMID: 31530641]
2.	Miyazaki, T. and Park, E.Y. Crystal structure of the Enterococcus faecalis α-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31. FEBS Lett. 594 (2020) 2282–2293. [DOI] [PMID: 32367553]
3.	Ikegaya, M., Miyazaki, T. and Park, E.Y. Biochemical characterization of Bombyx mori α-N-acetylgalactosaminidase belonging to the glycoside hydrolase family 31. Insect Mol Biol 30 (2021) 367–378. [DOI] [PMID: 33742736]
4.	Miyazaki, T., Ikegaya, M. and Alonso-Gil, S. Structural and mechanistic insights into the substrate specificity and hydrolysis of GH31 α-N-acetylgalactosaminidase. Biochimie (2021) . [DOI] [PMID: 34826537]

[EC 3.2.1.217 created 2022]