ExplorEnz: EC 3.2.1.227

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3.2.1.227

Accepted name:

N-acetyllactosaminidase

Reaction:

lacto-N-neotetraose + H₂O = N-acetyllactosamine + lactose

Glossary:

lacto-N-neotetraose = β-D-Gal-(1→4)-β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-D-Glc
N-acetyllactosamine = β-D-Gal-(1→4)-D-GlcNAc

Systematic name:

oligosaccharide exo β-(1,3)-N-acetyllactosamine hydrolase (non-reducing end)

Comments:

The enzyme, characterized from several bacterial species, hydrolyses the terminal N-acetyllactosamine residue (β-D-Gal-(1→4)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure β-D-Gal (1→4)-β-D-GlcNAc-(1→3)-β-D-Gal-(1→R). The enzyme also catalyses the activity of EC 3.2.1.140, lacto-N-biosidase, and is not active on oligosaccharides with a terminal GlcNAc residue.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

Vuillemin, M., Muschiol, J., Zhang, Y., Holck, J., Barrett, K., Morth, J.P., Meyer, A.S. and Zeuner, B. Discovery of lacto-N-biosidases and a novel N-acetyllactosaminidase activity in the CAZy family GH20: functional diversity and structural insights. Chembiochem :e202400710 (2024). [DOI] [PMID: 39239753]

[EC 3.2.1.227 created 2024]