Comments: |
A 50 kDa extracellular endopeptidase from Pseudomonas aeruginosa [1,2,6], Escherichia freundii [3], Serratia marcescens [4,5,6] and Erwinia chrysanthemi [7]. There is broad specificity in cleavage of the insulin B chain, with some species variations. The pH optimum for digesting various proteins is about 9 - 10. In peptidase family M10 (interstitial collagenase family). Formerly included in EC 3.4.24.4 |
References: |
1. |
Morihara, K., Tsuzuki, H. and Oka, T. Comparison of the specificities of various neutral proteinases from microorganisms. Arch. Biochem. Biophys. 123 (1968) 572–588. [DOI] [PMID: 4967801] |
2. |
Morihara, K., Tsuzuki, H. and Oka, T. On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides. Biochim. Biophys. Acta 309 (1973) 414–429. [DOI] [PMID: 4199986] |
3. |
Nakajima, M., Mizusawa, K. and Yoshida, F. Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii. Eur. J. Biochem. 44 (1974) 87–96. [DOI] [PMID: 4212288] |
4. |
Decedue, C.J., Broussard, E.A., II, L arson, A.D. and Braymer, H.D. Purification and characterization of the extracellular proteinase of Serratia marcescens. Biochim. Biophys. Acta 569 (1979) 293–301. [DOI] [PMID: 383155] |
5. |
Doerr, M. and Traub, W.H. Purification and characterization of two Serratia marcescens proteases. Zentralbl. Bakteriol., Mikrobiol. Hyg. Ser. A 257 (1984) 6–19. [PMID: 6380155] |
6. |
Nakahama, K., Yoshimura, K., Marumoto, R., Kikuchi, M., Lee, I.S., Hase, T. and Matsubara, H. Cloning and sequencing of Serratia protease gene. Nucleic Acids Res. 14 (1986) 5843–5856. [DOI] [PMID: 3016665] |
7. |
Dahler, G.S., Barras, F. and Keen, N.T. Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16. J. Bacteriol. 172 (1990) 5803–5815. [DOI] [PMID: 2211513] |
8. |
Okuda, K., Morihara, K., Atsumi, Y., Takeuchi, H., Kawamoto, S., Kawasaki, H., Suzuki, K. and Fukushima, J. Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455. Infect. Immun. 58 (1990) 4083–4088. [PMID: 2123832] |
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