The Enzyme Database

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Accepted name: N4-acetylcytidine amidohydrolase
Reaction: N4-acetylcytidine + H2O = cytidine + acetate
Other name(s): yqfB (gene name)
Systematic name: N4-acetylcytidine amidohydrolase
Comments: The enzyme from the bacterium Escherichia coli is one of the smallest known monomeric amidohydrolases (103-amino acids). The enzyme is active towards a wide range of N4-acylcytosines/cytidines, but is by far most active against N4-acetylcytidine.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB
1.  Shen, Y., Atreya, H.S., Liu, G. and Szyperski, T. G-matrix Fourier transform NOESY-based protocol for high-quality protein structure determination. J. Am. Chem. Soc. 127 (2005) 9085–9099. [PMID: 15969587]
2.  Stanislauskiene, R., Laurynenas, A., Rutkiene, R., Aucynaite, A., Tauraite, D., Meskiene, R., Urbeliene, N., Kaupinis, A., Valius, M., Kaliniene, L. and Meskys, R. YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N4-acylcytosine derivatives. Sci. Rep. 10:788 (2020). [PMID: 31964920]
[EC created 2020]

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