The Enzyme Database

Your query returned 1 entry.    printer_iconPrintable version

Accepted name: N-acyl-D-amino-acid deacylase
Reaction: N-acyl-D-amino acid + H2O = a carboxylate + D-amino acid
Systematic name: N-acyl-D-amino acid amidohydrolase
Comments: The enzyme from Alcaligenes denitrificans subsp. xylosoxydans and Alcaligenes xylosoxydans subsp. xylosoxydans has wide specificity; hydrolyses N-acyl derivative of neutral D-amino acids. Used in separating D- and L-amino acids. Requires zinc.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 65979-42-2
1.  Wakayama, M., Katsuno, Y., Hayashi, S., Miyamoto, Y., Sakai, K., Moriguchi, M. Cloning and sequencing of a gene encoding D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and expression of the gene in Escherichia coli. Biosci. Biotechnol. Biochem. 59 (1995) 2115–2119. [DOI] [PMID: 8541651]
2.  Wakayama, M., Hayashi, S., Yatsuda, Y., Katsuno, Y., Sakai, K. and Moriguchi, M. Overproduction of D-aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 in Escherichia coli and its purification. Protein Expr. Purif. 7 (1996) 395–399. [DOI] [PMID: 8776758]
[EC created 1999]

Data © 2001–2024 IUBMB
Web site © 2005–2024 Andrew McDonald