ExplorEnz: EC 3.5.1.89

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3.5.1.89

Accepted name:

N-acetylglucosaminylphosphatidylinositol deacetylase

Reaction:

6-(N-acetyl-α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H₂O = 6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate

For diagram of glycosylphosphatidyl-myo-inositol biosynthesis, click here

Other name(s):

N-acetyl-D-glucosaminylphosphatidylinositol acetylhydrolase; N-acetylglucosaminylphosphatidylinositol de-N-acetylase; GlcNAc-PI de-N-acetylase; GlcNAc-PI deacetylase; acetylglucosaminylphosphatidylinositol deacetylase

Systematic name:

6-(N-acetyl-α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase

Comments:

Involved in the second step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. The enzyme appears to be composed of a single subunit (PIG-L in mammalian cells and GPI12 in yeast). In some species, the long-chain sn-1-acyl group of the phosphatidyl group is replaced by a long-chain alkyl or alk-1-enyl group.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 122191-30-4

References:

1.	Doering, T.L., Masteron, W.J., Englund, P.T. and Hart, G.W. Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine. J. Biol. Chem. 264 (1989) 11168–11173. [PMID: 2525555]
2.	Nakamura, N., Inoue, N., Watanabe, R., Takahashi, M., Takeda, J., Stevens, V.L. and Kinoshita, T. Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase. J. Biol. Chem. 272 (1997) 15834–15840. [DOI] [PMID: 9188481]
3.	Watanabe, R., Ohishi, K., Maeda, Y., Nakamura, N. and Kinoshita, T. Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis. Biochem. J. 339 (1999) 185–192. [PMID: 10085243]
4.	Smith, T.K, Crossman, A., Borissow, C.N., Paterson, M.J., Dix, A., Brimacombe, J.S. and Ferguson, M.A.J. Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors. EMBO J. 20 (2001) 3322–3332. [DOI] [PMID: 11432820]

[EC 3.5.1.89 created 1992 as EC 3.1.1.69, transferred 2002 to EC 3.5.1.89, modified 2002]