ExplorEnz: EC 3.5.4.33

Your query returned 1 entry. Printable version

3.5.4.33

Accepted name:

tRNA(adenine³⁴) deaminase

Reaction:

adenine³⁴ in tRNA + H₂O = hypoxanthine³⁴ in tRNA + NH₃

Other name(s):

tRNA:A34 deaminase; tadA protein; ADAT2-ADAT3 complex; TADA; tRNA adenosine deaminase arginine; AtTadA; tadA/ecADAT2; tRNA A:34 deaminase

Systematic name:

tRNA(adenine³⁴) aminohydrolase

Comments:

The enzyme is involved in editing of tRNA. The active site contains Zn²⁺ [1].

Links to other databases:

BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB

References:

1.	Spears, J.L., Rubio, M.A., Gaston, K.W., Wywial, E., Strikoudis, A., Bujnicki, J.M., Papavasiliou, F.N. and Alfonzo, J.D. A single zinc ion is sufficient for an active Trypanosoma brucei tRNA editing deaminase. J. Biol. Chem. 286 (2011) 20366–20374. [DOI] [PMID: 21507956]
2.	Delannoy, E., Le Ret, M., Faivre-Nitschke, E., Estavillo, G.M., Bergdoll, M., Taylor, N.L., Pogson, B.J., Small, I., Imbault, P. and Gualberto, J.M. Arabidopsis tRNA adenosine deaminase arginine edits the wobble nucleotide of chloroplast tRNA^Arg(ACG) and is essential for efficient chloroplast translation. Plant Cell 21 (2009) 2058–2071. [DOI] [PMID: 19602623]
3.	Kuratani, M., Ishii, R., Bessho, Y., Fukunaga, R., Sengoku, T., Shirouzu, M., Sekine, S. and Yokoyama, S. Crystal structure of tRNA adenosine deaminase (TadA) from Aquifex aeolicus. J. Biol. Chem. 280 (2005) 16002–16008. [DOI] [PMID: 15677468]
4.	Wolf, J., Gerber, A.P. and Keller, W. tadA, an essential tRNA-specific adenosine deaminase from Escherichia coli. EMBO J. 21 (2002) 3841–3851. [DOI] [PMID: 12110595]
5.	Lee, W.H., Kim, Y.K., Nam, K.H., Priyadarshi, A., Lee, E.H., Kim, E.E., Jeon, Y.H., Cheong, C. and Hwang, K.Y. Crystal structure of the tRNA-specific adenosine deaminase from Streptococcus pyogenes. Proteins 68 (2007) 1016–1019. [DOI] [PMID: 17554781]
6.	Ragone, F.L., Spears, J.L., Wohlgamuth-Benedum, J.M., Kreel, N., Papavasiliou, F.N. and Alfonzo, J.D. The C-terminal end of the Trypanosoma brucei editing deaminase plays a critical role in tRNA binding. RNA 17 (2011) 1296–1306. [DOI] [PMID: 21602302]

[EC 3.5.4.33 created 2013]