| EC |
3.5.99.6 |
| Accepted name: |
glucosamine-6-phosphate deaminase |
| Reaction: |
α-D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3 |
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For diagram of UDP-N-acetylglucosamine biosynthesis, click here |
| Glossary: |
α-D-glucosamine 6-phosphate = 2-amino-2-deoxy-α-D-glucopyranose 6-phosphate |
| Other name(s): |
glucosaminephosphate isomerase (ambiguous); glucosamine-6-phosphate isomerase (ambiguous); phosphoglucosaminisomerase (ambiguous); glucosamine phosphate deaminase; aminodeoxyglucosephosphate isomerase (ambiguous); phosphoglucosamine isomerase (ambiguous); 2-amino-2-deoxy-D-glucose-6-phosphate aminohydrolase (ketol isomerizing) |
| Systematic name: |
2-amino-2-deoxy-α-D-glucose-6-phosphate aminohydrolase (ketol isomerizing) |
| Comments: |
The enzyme uses ring opening and isomerization of the aldose-ketose type to convert the -CH(-NH2)-CH=O group of glucosamine 6-phosphate into -C(=NH)-CH2-OH, forming 2-deoxy-2-imino-D-arabino-hexitol, which then hydrolyses to yield fructose 6-phosphate and ammonia. N-Acetyl-D-glucosamine 6-phosphate, which is not broken down, activates the enzyme. |
| Links to other databases: |
BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 9013-10-9 |
| References: |
| 1. |
Wolfe, J.B., Britton, B.B., Nakada, H.I. Glucosamine degradation by Escherichia coli. III. Isolation and studies of "phosphoglucosaminisomerase". Arch. Biochem. Biophys. 66 (1957) 333–339. [DOI] [PMID: 13403679] |
| 2. |
Comb, D.G., Roseman, S. Glucosamine metabolism. IV. Glucosamine-6-phosphate deaminase. J. Biol. Chem. 232 (1958) 807–827. [PMID: 13549465] |
| 3. |
Pattabiraman, T.N., Bachhawat, B.K. Purification of glucosamine 6-phosphate deaminase from human brain. Biochim. Biophys. Acta 54 (1961) 273–283. [DOI] [PMID: 14484386] |
| 4. |
Liu, C., Li, D., Liang, Y.H., Li, L.F. and Su, X.D. Ring-opening mechanism revealed by crystal structures of NagB and its ES intermediate complex. J. Mol. Biol. 379 (2008) 73–81. [DOI] [PMID: 18436239] |
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| [EC 3.5.99.6 created 1961 as EC 5.3.1.10, transferred 2000 to EC 3.5.99.6] |
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