ExplorEnz: EC 4.1.2.65

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4.1.2.65

Accepted name:

ferulate hydratase/lyase

Reaction:

ferulate + H₂O = vanillin + acetate (overall reaction)
(1a) ferulate + H₂O = 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoate
(1b) 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoate = vanillin + acetate

For diagram of vanillin biosynthesis, click here

Glossary:

ferulate = 4-hydroxy-3-methoxycinnamate
vanillin = 4-hydroxy-3-methoxybenzaldehyde

Other name(s):

vanillin synthase; VpVan; VAN; ferulate aldolase

Systematic name:

ferulate acetate-lyase (vanillin-forming)

Comments:

The enzyme is located in the chloroplasts of vanilla pods of the orchid Vanilla planifolia. It also converts ferulic acid 4-O-β-D-glucopyranoside to vanillin 4-O-β-D-glucopyranoside.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Gallage, N.J., Hansen, E.H., Kannangara, R., Olsen, C.E., Motawia, M.S., Jørgensen, K., Holme, I., Hebelstrup, K., Grisoni, M. and Møller, B.L. Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme. Nat. Commun. 5:4037 (2014). [DOI] [PMID: 24941968]
2.	Kundu, A. Vanillin biosynthetic pathways in plants. Planta 245 (2017) 1069–1078. [DOI] [PMID: 28357540]
3.	Gallage, N.J., Jørgensen, K., Janfelt, C., Nielsen, A.JZ., Naake, T., Dunski, E., Dalsten, L., Grisoni, M. and Møller, B.L. The intracellular localization of the vanillin biosynthetic machinery in pods of Vanilla planifolia. Plant Cell Physiol. 59 (2018) 304–318. [DOI] [PMID: 29186560]

[EC 4.1.2.65 created 2024]