| EC |
4.2.1.112 |
| Accepted name: |
acetylene hydratase |
| Reaction: |
acetaldehyde = acetylene + H2O |
| Other name(s): |
AH; acetaldehyde hydro-lyase |
| Systematic name: |
acetaldehyde hydro-lyase (acetylene-forming) |
| Comments: |
This is a non-redox-active enzyme that contains two molybdopterin guanine dinucleotide (MGD) cofactors, a tungsten centre and a cubane type [4Fe-4S] cluster [2].The tungsten centre binds a water molecule that is activated by an adjacent aspartate residue, enabling it to attack acetylene bound in a distinct hydrophobic pocket [2]. Ethylene cannot act as a substrate [1]. |
| Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 75788-81-7 |
| References: |
| 1. |
Rosner, B.M. and Schink, B. Purification and characterization of acetylene hydratase of Pelobacter acetylenicus, a tungsten iron-sulfur protein. J. Bacteriol. 177 (1995) 5767–5772. [DOI] [PMID: 7592321] |
| 2. |
Seiffert, G.B., Ullmann, G.M., Messerschmidt, A., Schink, B., Kroneck, P.M. and Einsle, O. Structure of the non-redox-active tungsten/[4Fe:4S] enzyme acetylene hydratase. Proc. Natl. Acad. Sci. USA 104 (2007) 3073–3077. [DOI] [PMID: 17360611] |
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| [EC 4.2.1.112 created 2007] |
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