ExplorEnz: EC 4.2.1.178

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4.2.1.178

Accepted name:

difructose-dianhydride-III synthase

Reaction:

inulobiose = α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride + H₂O

Glossary:

difructose anhydride III = α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride
inulobiose = β-D-fructofuranosyl-(2→1)-D-fructose

Other name(s):

DFA-IIIase; difructose anhydride III hydrolase

Systematic name:

inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride-forming)

Comments:

The enzyme participates in an inulin degradation pathway, in which it forms inulobiose from difructose anhydride III. A conformational change in the enzyme from the bacterium Pseudarthrobacter chlorophenolicus results in it also catalysing the activity of EC 4.2.2.18, inulin fructotransferase (DFA-III-forming).

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Tanaka, T., Uchiyama, T., Kobori, H. and Tanaka, K. Enzymic hydrolysis of di-D-fructofuranose 1, 2′; 2, 3′ dianhydride with Arthrobacter ureafaciens. J. Biochem. 78 (1975) 1201–1206. [DOI] [PMID: 1225919]
2.	Neubauer, A., Walter, M., and Buchholz, K. Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124. Biocatalysis and Biotransformation 18 (2000) 443–455. [DOI]
3.	Saito, K., Sumita, Y., Nagasaka, Y., Tomita, F. and Yokota, A. Molecular cloning of the gene encoding the di-D-fructofuranose 1,2′:2,3′ dianhydride hydrolysis enzyme (DFA IIIase) from Arthrobacter sp. H65-7. J. Biosci. Bioeng. 95 (2003) 538–540. [DOI] [PMID: 16233453]
4.	Yu, S., Wang, X., Zhang, T., Stressler, T., Fischer, L., Jiang, B. and Mu, W. Identification of a novel di-D-fructofuranose 1,2′:2,3′ dianhydride (DFA III) hydrolysis enzyme from Arthrobacter aurescens SK8.001. PLoS One 10:e0142640 (2015). [DOI] [PMID: 26555784]
5.	Yu, S., Shen, H., Cheng, Y., Zhu, Y., Li, X., and Mu, W. Structural and functional basis of difructose anhydride III hydrolase, which sequentially converts inulin using the same catalytic residue. ACS Catalysis 8 (2018) 10683–10697. [DOI]

[EC 4.2.1.178 created 2021]