ExplorEnz: EC 4.2.1.99

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4.2.1.99

Accepted name:

2-methylisocitrate dehydratase

Reaction:

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H₂O

Glossary:

cis-2-methylaconitate = (Z)-but-2-ene-1,2,3-tricarboxylate
(2S,3R)-2-methylisocitrate = (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = threo-D_s-2-methylisocitrate

Other name(s):

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase

Systematic name:

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]

Comments:

The enzyme from the fungus Yarrowia lipolytica (Saccharomycopsis) does not act on isocitrate.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 170780-51-5

References:

1.	Aoki, H., Uchiyama, H., Umetsu, H., Tabuchi, T. Isolation of 2-methylisocitrate dehydratase, a new enzyme serving in the methylcitric acid cycle for propionate metabolism, from Yarrowia lipolytica. Biosci. Biotechnol. Biochem. 59 (1995) 1825–1828.
2.	Tabuchi, T., Umetsu, H., Aoki, H., Uchiyama, H. Characteristics of 2-methylisocitrate dehydratase, isolated from Yarrowia lipolytica, in comparison to aconitase. Biosci. Biotechnol. Biochem. 59 (1995) 2013–2017.

[EC 4.2.1.99 created 1999]