The Enzyme Database

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Accepted name: L-serine ammonia-lyase
Reaction: L-serine = pyruvate + NH3 (overall reaction)
(1a) L-serine = 2-aminoprop-2-enoate + H2O
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous)
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Other name(s): serine deaminase; L-hydroxyaminoacid dehydratase; L-serine deaminase; L-serine dehydratase; L-serine hydro-lyase (deaminating)
Systematic name: L-serine ammonia-lyase (pyruvate-forming)
Comments: Most enzymes that catalyse this reaction are pyridoxal-phosphate-dependent, although some enzymes contain an iron-sulfur cluster instead [6]. The reaction catalysed by both types of enzymes involves the initial elimination of water to form an enamine intermediate (hence the enzyme’s original classification as EC, L-serine dehydratase), followed by tautomerization to an imine form and hydrolysis of the C-N bond. The latter reaction, which can occur spontaneously, is also be catalysed by EC, 2-iminobutanoate/2-iminopropanoate deaminase. This reaction is also carried out by EC, threonine ammonia-lyase, from a number of sources.
Links to other databases: BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 9014-27-1
1.  Ramos, F. and Wiame, J.-M. Occurrence of a catabolic L-serine (L-threonine) deaminase in Saccharomyces cerevisiae. Eur. J. Biochem. 123 (1982) 571–576. [DOI] [PMID: 7042346]
2.  Simon, D., Hoshino, J. and Kröger, H. L-Serine dehydratase from rat liver. Purification and some properties. Biochim. Biophys. Acta 321 (1973) 361–368. [DOI] [PMID: 4750769]
3.  Suda, M. and Nakagawa, H. L-Serine dehydratase (rat liver). Methods Enzymol. 17B (1971) 346–351.
4.  Sagers, R.D. and Carter, J. E. L-Serine dehydratase (Clostridium acidiurica). Methods Enzymol. 17B (1971) 351–356.
5.  Robinson, W.G. and Labow, R. L-Serine dehydratase (Escherichia coli). Methods Enzymol. 17B (1971) 356–360.
6.  Grabowski, R., Hofmeister, A.E. and Buckel, W. Bacterial L-serine dehydratases: a new family of enzymes containing iron-sulfur clusters. Trends Biochem. Sci. 18 (1993) 297–300. [DOI] [PMID: 8236444]
7.  Yamada, T., Komoto, J., Takata, Y., Ogawa, H., Pitot, H.C. and Takusagawa, F. Crystal structure of serine dehydratase from rat liver. Biochemistry 42 (2003) 12854–12865. [DOI] [PMID: 14596599]
[EC created 1961 as EC, transferred 2001 to EC, modified 2014]

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