| EC |
4.4.1.44 |
| Accepted name: |
2-(S-pantetheinyl)-carbapenam-3-carboxylate synthase |
| Reaction: |
pantetheine + (3S,5S)-carbapenam-3-carboxylate + S-adenosyl-L-methionine = (2R,3R,5R)-2-(S-pantetheinyl)-carbapenam-3-carboxylate + 5′-deoxyadenosine + L-methionine |
| Other name(s): |
thnL (gene name); (2R,3R,5S)-2-(S-pantetheinyl)-carbapenam-3-carboxylate lyase |
| Systematic name: |
(2R,3R,5R)-2-(S-pantetheinyl)-carbapenam-3-carboxylate lyase |
| Comments: |
The enzyme uses radical SAM (AdoMet) chemistry to catalyse thioether bond formation between pantetheine and C-2 of the carbapenam precursor (3S,5S)-carbapenam-3-carboxylate leading to formation of complex carbapenems. Contains a bound [4Fe-4S] cluster and hydroxocobalamin. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Sinner, E.K., Li, R., Marous, D.R. and Townsend, C.A. ThnL, a B12-dependent radical S-adenosylmethionine enzyme, catalyzes thioether bond formation in carbapenem biosynthesis. Proc. Natl. Acad. Sci. USA 119:e2206494119 (2022). [DOI] [PMID: 35969793] |
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| [EC 4.4.1.44 created 2025] |
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