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Your query returned 1 entry. Printable version
EC | 4.6.1.21 | ||||
Accepted name: | Enterobacter ribonuclease | ||||
Reaction: | RNA containing adenosine-cytidine + H2O = an [RNA fragment]-3′-cytidine-3′-phosphate + a 5′-a hydroxy-adenosine -3′-[RNA fragment] (overall reaction) (1a) RNA containing adenosine-cytidine = an [RNA fragment]-3′-cytidine-2′,3′-cyclophosphate + a 5′-a hydroxy-adenosine -3′-[RNA fragment] (1b) an [RNA fragment]-3′-cytidine-2′,3′-cyclophosphate + H2O = an [RNA fragment]-3′-cytidine-3′-phosphate |
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Systematic name: | [RNA]-adenosine-cytidine 5′-hydroxy-adenosoine ribonucleotide-3′-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3′-cytidine-2′,3′-cyclophosphate-forming and hydrolysing) | ||||
Comments: | Preference for cleavage at Cp-A bonds. Homopolymers of A, U or G are not hydrolysed. CpG bonds are hydrolysed less well and there is no detectable hydrolysis between two purines or two pyrimidines. The enzyme catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5′-hydroxy-phosphooligonucletides and 3′-phosphooligonucleotides ending a with 2′,3′-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3′,5′-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5′-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3′-nucleotides. | ||||
Links to other databases: | BRENDA, EXPASY, Gene, KEGG, MetaCyc, PDB, CAS registry number: 395640-99-0 | ||||
References: |
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