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Your query returned 1 entry. 
Printable version
| EC | 4.6.1.24 | ||||||
| Accepted name: | ribonuclease T1 | ||||||
| Reaction: | [RNA] containing guanosine + H2O = an [RNA fragment]-3′-guanosine-3′-phosphate + a 5′-hydroxy-ribonucleotide-3′-[RNA fragment] (overall reaction) (1a) [RNA] containing guanosine = [RNA fragment]-3′-guanosine-2′,3′-cyclophosphate + a 5′-hydroxy-ribonucleotide-3′-[RNA fragment] (1b) [RNA fragment]-3′-guanosine-2′,3′-cyclophosphate + H2O = [RNA fragment]-3′-guanosine-3′-phosphate |
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| Other name(s): | barnase; bacterial ribonuclease Sa; guanyloribonuclease; Aspergillus oryzae ribonuclease; RNase N1; RNase N2; ribonuclease N3; ribonuclease U1; ribonuclease F1; ribonuclease Ch; ribonuclease PP1; ribonuclease SA; RNase F1; ribonuclease C2; binase; RNase Sa; guanyl-specific RNase; RNase G; RNase T1; ribonuclease guaninenucleotido-2′-transferase (cyclizing); ribonuclease N1 | ||||||
| Systematic name: | [RNA]-guanosine 5′-hydroxy-ribonucleotide-3′-[RNA fragment]-lyase (cyclicizing; [RNA fragment]-3′-guanosine-2′,3′-cyclophosphate-forming and hydrolysing) | ||||||
| Comments: | A family of related enzymes found in some fungi and bacteria. The enzyme is specific for cleavage at the 3′-phosphate group of guanosine in single stranded RNA, and catalyses a two-stage endonucleolytic cleavage. The first reaction produces 5′-hydroxy-phosphooligonucletides and 3′-phosphooligonucleotides ending in Gp with 2′,3′-cyclic phosphodiester, which are released from the enzyme. The enzyme then hydrolyses these cyclic compounds in a second reaction that takes place only when all the susceptible 3′,5′-phosphodiester bonds have been cyclised. The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5′-hydroxyl group of ribose. The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3′-nucleotides. | ||||||
| Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||
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