The Enzyme Database

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EC 5.1.3.31     
Accepted name: D-tagatose 3-epimerase
Reaction: (1) D-tagatose = D-sorbose
(2) D-psicose = D-fructose
For diagram of tagatose metabolism, click here
Glossary: D-psicose = D-ribo-hex-2-ulose
Other name(s): L-ribulose 3-epimerase; ketose 3-epimerase
Systematic name: D-tagatose 3-epimerase
Comments: The enzymes isolated from the bacteria Pseudomonas cichorii [2], Pseudomonas sp. ST-24 [1], Rhodobacter sphaeroides [3] and Mesorhizobium loti [4] catalyse the epimerization of various ketoses at the C-3 position, interconverting D-fructose and D-psicose, D-tagatose and D-sorbose, D-ribulose and D-xylulose, and L-ribulose and L-xylulose. The specificity depends on the species. The enzymes from Pseudomonas cichorii and Rhodobacter sphaeroides require Mn2+ [2,3].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
References:
1.  Itoh, H., Okaya, H., Khan, A. R., Tajima, S., Hayakawa, S., Izumori, K. Purification and characterization of D-tagatose 3-epimerase from Pseudomonas sp. ST-24. Biosci. Biotechnol. Biochem. 58 (1994) 2168–2171.
2.  Yoshida, H., Yamada, M., Nishitani, T., Takada, G., Izumori, K. and Kamitori, S. Crystal structures of D-tagatose 3-epimerase from Pseudomonas cichorii and its complexes with D-tagatose and D-fructose. J. Mol. Biol. 374 (2007) 443–453. [DOI] [PMID: 17936787]
3.  Zhang, L., Mu, W., Jiang, B. and Zhang, T. Characterization of D-tagatose-3-epimerase from Rhodobacter sphaeroides that converts D-fructose into D-psicose. Biotechnol. Lett. 31 (2009) 857–862. [DOI] [PMID: 19205890]
4.  Uechi, K., Takata, G., Fukai, Y., Yoshihara, A. and Morimoto, K. Gene cloning and characterization of L-ribulose 3-epimerase from Mesorhizobium loti and its application to rare sugar production. Biosci. Biotechnol. Biochem. 77 (2013) 511–515. [DOI] [PMID: 23470755]
[EC 5.1.3.31 created 2014]
 
 


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