The Enzyme Database

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Accepted name: 16S rRNA pseudouridine516 synthase
Reaction: 16S rRNA uridine516 = 16S rRNA pseudouridine516
Other name(s): 16S RNA pseudouridine516 synthase; 16S PsiI516 synthase; 16S RNA Ψ516 synthase; RNA pseudouridine synthase RsuA; RsuA; 16S RNA pseudouridine 516 synthase
Systematic name: 16S rRNA-uridine516 uracil mutase
Comments: The enzyme is specific for uridine516 in 16S rRNA. In vitro, the enzyme does not modify free 16S rRNA. The preferred substrate is a 5′-terminal fragment of 16S rRNA complexed with 30S ribosomal proteins [1].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, PDB
1.  Wrzesinski, J., Bakin, A., Nurse, K., Lane, B.G. and Ofengand, J. Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli. Biochemistry 34 (1995) 8904–8913. [PMID: 7612632]
2.  Conrad, J., Niu, L., Rudd, K., Lane, B.G. and Ofengand, J. 16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases. RNA 5 (1999) 751–763. [PMID: 10376875]
3.  Sivaraman, J., Sauve, V., Larocque, R., Stura, E.A., Schrag, J.D., Cygler, M. and Matte, A. Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP. Nat. Struct. Biol. 9 (2002) 353–358. [DOI] [PMID: 11953756]
[EC created 2011]

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