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Your query returned 1 entry. Printable version
EC | 6.3.5.4 | ||||||||||||
Accepted name: | asparagine synthase (glutamine-hydrolysing) | ||||||||||||
Reaction: | ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (overall reaction) (1a) L-glutamine + H2O = L-glutamate + NH3 (1b) ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine |
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Other name(s): | asparagine synthetase (glutamine-hydrolysing); glutamine-dependent asparagine synthetase; asparagine synthetase B; AS; AS-B | ||||||||||||
Systematic name: | L-aspartate:L-glutamine amido-ligase (AMP-forming) | ||||||||||||
Comments: | The enzyme from Escherichia coli has two active sites [4] that are connected by an intramolecular ammonia tunnel [5,6]. The enzyme catalyses three distinct chemical reactions: glutamine hydrolysis to yield ammonia takes place in the N-terminal domain. The C-terminal active site mediates both the synthesis of a β-aspartyl-AMP intermediate and its subsequent reaction with ammonia. The ammonia released is channeled to the other active site to yield asparagine [6]. | ||||||||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37318-72-2 | ||||||||||||
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