EC |
6.3.5.9 |
Accepted name: |
hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) |
Reaction: |
2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate |
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For diagram of corrin biosynthesis (part 4), click here |
Other name(s): |
CobB |
Systematic name: |
hydrogenobyrinic-acid:L-glutamine amido-ligase (AMP-forming) |
Comments: |
This enzyme, which participates in the aerobic (late cobalt insertion) cobalamin biosynthesis pathway, generates hydrogenobyrinate a,c-diamide, the substrate required by EC 6.6.1.2, cobaltochelatase, which adds cobalt to the macrocycle. The equivalent reaction in the anaerobic cobalamin biosynthesis pathway is catalysed by EC 6.3.5.11, cobyrinate a,c-diamide synthase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 132053-22-6 |
References: |
1. |
Debussche, L., Thibaut, D., Cameron, B., Crouzet, J. and Blanche, F. Purification and characterization of cobyrinic acid a,c-diamide synthase from Pseudomonas denitrificans. J. Bacteriol. 172 (1990) 6239–6244. [DOI] [PMID: 2172209] |
2. |
Warren, M.J., Raux, E., Schubert, H.L. and Escalante-Semerena, J.C. The biosynthesis of adenosylcobalamin (vitamin B12). Nat. Prod. Rep. 19 (2002) 390–412. [PMID: 12195810] |
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[EC 6.3.5.9 created 2004] |
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