EC |
4.2.1.127 |
Accepted name: |
linalool dehydratase |
Reaction: |
(3S)-linalool = myrcene + H2O |
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For diagram of acyclic monoterpenoid biosynthesis, click here |
Glossary: |
linalool = 3,7-dimethylocta-1,6-dien-3-ol |
Other name(s): |
linalool hydro-lyase (myrcene-forming) |
Systematic name: |
(3S)-linalool hydro-lyase (myrcene-forming) |
Comments: |
In absence of oxygen the bifunctional linalool dehydratase-isomerase can catalyse in vitro two reactions, the hydration of myrcene to (3S)-linalool and the isomerization of (3S)-linalool to geraniol, the latter activity being classified as EC 5.4.4.4, geraniol isomerase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Brodkorb, D., Gottschall, M., Marmulla, R., Lüddeke, F. and Harder, J. Linalool dehydratase-isomerase, a bifunctional enzyme in the anaerobic degradation of monoterpenes. J. Biol. Chem. 285 (2010) 30436–30442. [DOI] [PMID: 20663876] |
2. |
Lüddeke, F. and Harder, J. Enantiospecific (S)-(+)-linalool formation from β-myrcene by linalool dehydratase-isomerase. Z. Naturforsch. C 66 (2011) 409–412. [PMID: 21950166] |
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[EC 4.2.1.127 created 2011, modified 2012] |
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EC |
4.2.3.25 |
Accepted name: |
S-linalool synthase |
Reaction: |
geranyl diphosphate + H2O = (3S)-linalool + diphosphate |
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For diagram of acyclic monoterpenoid biosynthesis, click here |
Glossary: |
(3S)-linalool = (3S)-3,7-dimethylocta-1,6-dien-3-ol |
Other name(s): |
LIS; Lis; 3S-linalool synthase |
Systematic name: |
geranyl-diphosphate diphosphate-lyase [(3S)-linalool-forming] |
Comments: |
Requires Mn2+ or Mg2+ for activity. Neither (S)- nor (R)-linalyl diphosphate can act as substrate for the enzyme from the flower Clarkia breweri [1]. Unlike many other monoterpene synthases, only a single product, (3S)-linalool, is formed. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 160477-81-6 |
References: |
1. |
Pichersky, E., Lewinsohn, E. and Croteau, R. Purification and characterization of S-linalool synthase, an enzyme
involved in the production of floral scent in Clarkia breweri. Arch. Biochem. Biophys. 316 (1995) 803–807. [DOI] [PMID: 7864636] |
2. |
Lücker, J., Bouwmeester, H.J., Schwab, W., Blaas, J., van der Plas, L.H. and Verhoeven, H.A. Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-D-glucopyranoside. Plant J. 27 (2001) 315–324. [DOI] [PMID: 11532177] |
3. |
Dudareva, N., Cseke, L., Blanc, V.M. and Pichersky, E. Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell 8 (1996) 1137–1148. [DOI] [PMID: 8768373] |
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[EC 4.2.3.25 created 2006] |
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EC |
5.4.4.4 |
Accepted name: |
geraniol isomerase |
Reaction: |
geraniol = (3S)-linalool |
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For diagram of acyclic monoterpenoid biosynthesis, click here |
Systematic name: |
geraniol hydroxymutase |
Comments: |
In absence of oxygen the bifunctional linalool dehydratase-isomerase can catalyse in vitro two reactions, the isomerization of (3S)-linalool to geraniol and the hydration of myrcene to (3S)-linalool, the latter activity being classified as EC 4.2.1.127, linalool dehydratase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Brodkorb, D., Gottschall, M., Marmulla, R., Lüddeke, F. and Harder, J. Linalool dehydratase-isomerase, a bifunctional enzyme in the anaerobic degradation of monoterpenes. J. Biol. Chem. 285 (2010) 30436–30442. [DOI] [PMID: 20663876] |
2. |
Lüddeke, F. and Harder, J. Enantiospecific (S)-(+)-linalool formation from β-myrcene by linalool dehydratase-isomerase. Z. Naturforsch. C 66 (2011) 409–412. [PMID: 21950166] |
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[EC 5.4.4.4 created 2011, modified 2012] |
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