EC |
1.1.1.379 |
Accepted name: |
(R)-mandelate dehydrogenase |
Reaction: |
(R)-mandelate + NAD+ = phenylglyoxylate + NADH + H+ |
Glossary: |
(R)-mandelate = D-mandelate |
Other name(s): |
ManDH2; D-ManDH2; D-mandelate dehydrogenase (ambiguous) |
Systematic name: |
(R)-mandelate:NAD+ 2-oxidoreductase |
Comments: |
The enzyme, found in bacteria and fungi, can also accept a number of substituted mandelate derivatives, such as 3-hydroxymandelate, 4-hydroxymandelate, 2-methoxymandelate, 4-hydroxy-3-methoxymandelate and 3-hydroxy-4-methoxymandelate. The enzyme has no activity with (S)-mandelate (cf. EC 1.1.99.31, (S)-mandelate dehydrogenase) [1,2]. The enzyme transfers the pro-R-hydrogen from NADH [2]. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Baker, D.P. and Fewson, C.A. Purification and characterization of D(–)-mandelate dehydrogenase from Rhodotorula graminis. Microbiology 135 (1989) 2035–2044. |
2. |
Baker, D.P., Kleanthous, C., Keen, J.N., Weinhold, E. and Fewson, C.A. Mechanistic and active-site studies on D(–)-mandelate dehydrogenase from Rhodotorula graminis. Biochem. J. 281 (1992) 211–218. [PMID: 1731758] |
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[EC 1.1.1.379 created 2014] |
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EC |
3.5.1.86 |
Accepted name: |
mandelamide amidase |
Reaction: |
(R)-mandelamide + H2O = (R)-mandelate + NH3 |
Glossary: |
(R)-mandelate = (R)-2-hydroxy-2-phenylacetate
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Other name(s): |
Pseudomonas mandelamide hydrolase |
Systematic name: |
mandelamide hydrolase |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Yamamoto, K., Oishi, K., Fujimatsu, I. and Komatsu, K. Production of R-(-)-mandelic acid from mandelonitrile by Alcaligenes faecalis ATCC 8750. Appl. Environ. Microbiol. 57 (1991) 3028–3032. [PMID: 1660699] |
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[EC 3.5.1.86 created 2000] |
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EC |
5.1.2.2 |
Accepted name: |
mandelate racemase |
Reaction: |
(S)-mandelate = (R)-mandelate |
Glossary: |
mandelate = 2-hydroxy-2-phenylacetate
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Systematic name: |
mandelate racemase |
Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9024-04-8 |
References: |
1. |
Gunsalus, C.F., Stanier, R.Y. and Gunsalus, I.C. The enzymatic conversion of mandelic acid to benzoic acid. III. Fractionation and properties of the soluble enzymes. J. Bacteriol. 66 (1953) 548–553. [PMID: 13108854] |
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[EC 5.1.2.2 created 1961] |
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