EC |
1.14.13.209 |
Accepted name: |
salicyloyl-CoA 5-hydroxylase |
Reaction: |
2-hydroxybenzoyl-CoA + NADH + H+ + O2 = gentisyl-CoA + NAD+ + H2O |
Glossary: |
2-hydroxybenzoyl-CoA = salicyloyl-CoA
gentisate = 2,5-dihydroxybenzoate |
Other name(s): |
sdgC (gene name) |
Systematic name: |
salicyloyl-CoA,NADH:oxygen oxidoreductase (5-hydroxylating) |
Comments: |
The enzyme, characterized from the bacterium Streptomyces sp. WA46, participates in a pathway for salicylate degradation. cf. EC 1.14.13.172, salicylate 5-hydroxylase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Ishiyama, D., Vujaklija, D. and Davies, J. Novel pathway of salicylate degradation by Streptomyces sp. strain WA46. Appl. Environ. Microbiol. 70 (2004) 1297–1306. [DOI] [PMID: 15006746] |
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[EC 1.14.13.209 created 2015] |
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EC |
2.3.1.177 |
Accepted name: |
3,5-dihydroxybiphenyl synthase |
Reaction: |
3 malonyl-CoA + benzoyl-CoA = 4 CoA + 3,5-dihydroxybiphenyl + 4 CO2 |
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For diagram of polyketides biosynthesis, click here |
Other name(s): |
BIS1; biphenyl synthase (ambiguous) |
Systematic name: |
malonyl-CoA:benzoyl-CoA malonyltransferase |
Comments: |
A polyketide synthase that is involved in the production of the phytoalexin aucuparin. 2-Hydroxybenzoyl-CoA can also act as substrate but it leads to the derailment product 4-hydroxycoumarin (cf. EC 2.3.1.208, 4-hydroxycoumarin synthase) [2]. This enzyme uses the same starter substrate as EC 2.3.1.151, benzophenone synthase. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 1217551-24-0 |
References: |
1. |
Liu, B., Beuerle, T., Klundt, T. and Beerhues, L. Biphenyl synthase from yeast-extract-treated cell cultures of Sorbus aucuparia. Planta 218 (2004) 492–496. [DOI] [PMID: 14595561] |
2. |
Liu, B., Raeth, T., Beuerle, T. and Beerhues, L. Biphenyl synthase, a novel type III polyketide synthase. Planta 225 (2007) 1495–1503. [DOI] [PMID: 17109150] |
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[EC 2.3.1.177 created 2006, modified 2012] |
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EC |
2.3.1.208 |
Accepted name: |
4-hydroxycoumarin synthase |
Reaction: |
malonyl-CoA + 2-hydroxybenzoyl-CoA = 2 CoA + 4-hydroxycoumarin + CO2 |
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For diagram of polyketides biosynthesis, click here |
Glossary: |
2-hydroxybenzoyl-CoA = salicyloyl-CoA |
Other name(s): |
BIS2; BIS3 |
Systematic name: |
malonyl-CoA:2-hydroxybenzoyl-CoA malonyltransferase |
Comments: |
The enzyme, a polyketide synthase, can also accept benzoyl-CoA as substrate, which it condenses with 3 malonyl-CoA molecules to form 3,5-dihydroxybiphenyl (cf. EC 2.3.1.177, biphenyl synthase) [1]. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Liu, B., Raeth, T., Beuerle, T. and Beerhues, L. A novel 4-hydroxycoumarin biosynthetic pathway. Plant Mol. Biol. 72 (2010) 17–25. [DOI] [PMID: 19757094] |
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[EC 2.3.1.208 created 2012] |
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EC |
6.2.1.65 |
Accepted name: |
salicylate—CoA ligase |
Reaction: |
ATP + salicylate + CoA = AMP + diphosphate + 2-hydroxybenzoyl-CoA (overall reaction) (1a) ATP + salicylate = diphosphate + (2-hydroxybenzoyl)adenylate (1b) (2-hydroxybenzoyl)adenylate + CoA = AMP + 2-hydroxybenzoyl-CoA |
Glossary: |
2-hydroxybenzoyl-CoA = salicyloyl-CoA |
Other name(s): |
sdgA (gene name) |
Systematic name: |
salicylate:CoA ligase (AMP-forming) |
Comments: |
The enzyme, characterized from the bacteria Thauera aromatica and Streptomyces sp. WA46, participates in a salicylate degradation pathway. It activates salicylate by its adenylation to (2-hydroxybenzoyl)adenylate, followed by the transfer of the activated compound to coenzyme A. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Bonting, C.F. and Fuchs, G. Anaerobic metabolism of 2-hydroxybenzoic acid (salicylic acid) by a denitrifying bacterium. Arch. Microbiol. 165 (1996) 402–408. [PMID: 8661934] |
2. |
Ishiyama, D., Vujaklija, D. and Davies, J. Novel pathway of salicylate degradation by Streptomyces sp. strain WA46. Appl. Environ. Microbiol. 70 (2004) 1297–1306. [DOI] [PMID: 15006746] |
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[EC 6.2.1.65 created 2020] |
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