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3.6.1.62

Accepted name:

5′-(N⁷-methylguanosine 5′-triphospho)-[mRNA] hydrolase

Reaction:

a 5′-(N⁷-methylguanosine 5′-triphospho)-[mRNA] + H₂O = N⁷-methylguanosine 5′-diphosphate + a 5′-phospho-[mRNA]

Glossary:

N⁷-methylguanosine 5′-diphosphate = m7GDP

Other name(s):

Dcp2; NUDT16; D10 protein; D9 protein; D10 decapping enzyme; decapping enzyme; m⁷GpppN-mRNA hydrolase; m⁷GpppN-mRNA m⁷GDP phosphohydrolase

Systematic name:

5′-(N⁷-methylguanosine 5′-triphospho)-[mRNA] N⁷-methylguanosine-5′-diphosphate phosphohydrolase

Comments:

Decapping of mRNA is a critical step in eukaryotic mRNA turnover. The enzyme is unable to cleave a free cap structure (m⁷GpppG) [3]. The enzyme from Vaccinia virus is synergistically activated in the presence of Mg²⁺ and Mn²⁺ [5].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Xu, J., Yang, J.Y., Niu, Q.W. and Chua, N.H. Arabidopsis DCP2, DCP1, and VARICOSE form a decapping complex required for postembryonic development. Plant Cell 18 (2006) 3386–3398. [DOI] [PMID: 17158604]
2.	Lu, G., Zhang, J., Li, Y., Li, Z., Zhang, N., Xu, X., Wang, T., Guan, Z., Gao, G.F. and Yan, J. hNUDT16: a universal decapping enzyme for small nucleolar RNA and cytoplasmic mRNA. Protein Cell 2 (2011) 64–73. [DOI] [PMID: 21337011]
3.	van Dijk, E., Cougot, N., Meyer, S., Babajko, S., Wahle, E. and Seraphin, B. Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures. EMBO J. 21 (2002) 6915–6924. [DOI] [PMID: 12486012]
4.	Parrish, S., Resch, W. and Moss, B. Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression. Proc. Natl. Acad. Sci. USA 104 (2007) 2139–2144. [DOI] [PMID: 17283339]
5.	Souliere, M.F., Perreault, J.P. and Bisaillon, M. Characterization of the vaccinia virus D10 decapping enzyme provides evidence for a two-metal-ion mechanism. Biochem. J. 420 (2009) 27–35. [DOI] [PMID: 19210265]
6.	Parrish, S. and Moss, B. Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. J. Virol. 81 (2007) 12973–12978. [DOI] [PMID: 17881455]
7.	Song, M.G., Li, Y. and Kiledjian, M. Multiple mRNA decapping enzymes in mammalian cells. Mol. Cell 40 (2010) 423–432. [DOI] [PMID: 21070968]

[EC 3.6.1.62 created 2012, modified 2013]