The Enzyme Database

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EC 2.3.1.245     
Accepted name: 3-hydroxy-5-phosphooxypentane-2,4-dione thiolase
Reaction: glycerone phosphate + acetyl-CoA = 3-hydroxy-2,4-dioxopentyl phosphate + CoA
Glossary: (4S)-4,5-dihydroxypentane-2,3-dione = autoinducer 2 = AI-2
Other name(s): lsrF (gene name); 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase
Systematic name: acetyl-CoA:glycerone phosphate C-acetyltransferase
Comments: The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Diaz, Z., Xavier, K.B. and Miller, S.T. The crystal structure of the Escherichia coli autoinducer-2 processing protein LsrF. PLoS One 4:e6820 (2009). [DOI] [PMID: 19714241]
2.  Marques, J.C., Oh, I.K., Ly, D.C., Lamosa, P., Ventura, M.R., Miller, S.T. and Xavier, K.B. LsrF, a coenzyme A-dependent thiolase, catalyzes the terminal step in processing the quorum sensing signal autoinducer-2. Proc. Natl. Acad. Sci. USA 111 (2014) 14235–14240. [DOI] [PMID: 25225400]
[EC 2.3.1.245 created 2015, modified 2021]
 
 
EC 2.7.1.189     
Accepted name: autoinducer-2 kinase
Reaction: ATP + (S)-4,5-dihydroxypentane-2,3-dione = ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
Glossary: (S)-4,5-dihydroxypentane-2,3-dione = autoinducer 2 = AI-2
Other name(s): lsrK (gene name)
Systematic name: ATP:(S)-4,5-dihydroxypentane-2,3-dione 5-phosphotransferase
Comments: The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Xavier, K.B., Miller, S.T., Lu, W., Kim, J.H., Rabinowitz, J., Pelczer, I., Semmelhack, M.F. and Bassler, B.L. Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria. ACS Chem. Biol. 2 (2007) 128–136. [DOI] [PMID: 17274596]
2.  Roy, V., Fernandes, R., Tsao, C.Y. and Bentley, W.E. Cross species quorum quenching using a native AI-2 processing enzyme. ACS Chem. Biol. 5 (2010) 223–232. [DOI] [PMID: 20025244]
3.  Zhu, J., Hixon, M.S., Globisch, D., Kaufmann, G.F. and Janda, K.D. Mechanistic insights into the LsrK kinase required for autoinducer-2 quorum sensing activation. J. Am. Chem. Soc. 135 (2013) 7827–7830. [DOI] [PMID: 23672516]
[EC 2.7.1.189 created 2015]
 
 
EC 5.3.1.32     
Accepted name: (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione isomerase
Reaction: (2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate
Glossary: (2S)-2-hydroxy-3,4-dioxopentyl phosphate = (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione
(4S)-4,5-dihydroxypentane-2,3-dione = autoinducer 2 = AI-2
Other name(s): lsrG (gene name); phospho-AI-2 isomerase; (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione aldose-ketose-isomerase; (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase; (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione aldose-ketose-isomerase
Systematic name: (2S)-2-hydroxy-3,4-dioxopentyl phosphate aldose-ketose-isomerase
Comments: The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Xavier, K.B., Miller, S.T., Lu, W., Kim, J.H., Rabinowitz, J., Pelczer, I., Semmelhack, M.F. and Bassler, B.L. Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria. ACS Chem. Biol. 2 (2007) 128–136. [DOI] [PMID: 17274596]
2.  Marques, J.C., Lamosa, P., Russell, C., Ventura, R., Maycock, C., Semmelhack, M.F., Miller, S.T. and Xavier, K.B. Processing the interspecies quorum-sensing signal autoinducer-2 (AI-2): characterization of phospho-(S)-4,5-dihydroxy-2,3-pentanedione isomerization by LsrG protein. J. Biol. Chem. 286 (2011) 18331–18343. [DOI] [PMID: 21454635]
[EC 5.3.1.32 created 2015]
 
 
EC 7.6.2.13     
Accepted name: ABC-type autoinducer-2 transporter
Reaction: ATP + H2O + (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran-[AI-2-binding protein][side 1] = ADP + phosphate + (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran[side 2] + [AI-2-binding protein][side 1]
Glossary: autoinducer-2 = AI-2 = (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran
Other name(s): autoinducer-2 transporting ATPase; autoinducer-2 ABC transporter; LsrACDB (gene names)
Systematic name: ATP phosphohydrolase (ABC-type, AI-2 importing)
Comments: ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. A bacterial enzyme that interacts with an extracytoplasmic substrate binding protein and mediates the uptake of the signalling molecule (2R,4S)-2-methyl-2,3,3,4-tetrahydoxytetrahydrofuran (also known as autoinducer-2).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Taga, M.E., Semmelhack, J.L. and Bassler, B.L. The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium. Mol. Microbiol. 42 (2001) 777–793. [PMID: 11722742]
2.  Xavier, K.B. and Bassler, B.L. Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli. J. Bacteriol. 187 (2005) 238–248. [PMID: 15601708]
[EC 7.6.2.13 created 2019]
 
 


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