The Enzyme Database

Your query returned 4 entries.    printer_iconPrintable version

Accepted name: 3α-hydroxyglycyrrhetinate dehydrogenase
Reaction: 3α-hydroxyglycyrrhetinate + NADP+ = 3-oxoglycyrrhetinate + NADPH + H+
Systematic name: 3α-hydroxyglycyrrhetinate:NADP+ 3-oxidoreductase
Comments: Highly specific to 3α-hydroxy derivatives of glycyrrhetinate and its analogues. Not identical to EC 3α-hydroxysteroid dehydrogenase (Si-specific).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 114308-07-5
1.  Akao, T., Akao, T., Hattori, M., Namba, T. and Kobashi, K. Purification and properties of 3α-hydroxyglycyrrhetinate dehydrogenase of Clostridium innocuum from human intestine. J. Biochem. (Tokyo) 103 (1988) 504–507. [PMID: 3164718]
[EC created 1990]
Transferred entry: 11-oxo-β-amyrin 30-oxidase. Now EC, 11-oxo-β-amyrin 30-oxidase.
[EC created 2013, deleted 2018]
Accepted name: 11-oxo-β-amyrin 30-oxidase
Reaction: 11-oxo-β-amyrin + 3 O2 + 3 [reduced NADPH—hemoprotein reductase] = glycyrrhetinate + 3 [oxidized NADPH—hemoprotein reductase] + 4 H2O (overall reaction)
(1a) 11-oxo-β-amyrin + O2 + [reduced NADPH—hemoprotein reductase] = 30-hydroxy-11-oxo-β-amyrin + [oxidized NADPH—hemoprotein reductase] + H2O
(1b) 30-hydroxy-11-oxo-β-amyrin + O2 + [reduced NADPH—hemoprotein reductase] = glycyrrhetaldehyde + [oxidized NADPH—hemoprotein reductase] + 2 H2O
(1c) glycyrrhetaldehyde + O2 + [reduced NADPH—hemoprotein reductase] = glycyrrhetinate + [oxidized NADPH—hemoprotein reductase] + H2O
For diagram of glycyrrhenate biosynthesis, click here
Other name(s): CYP72A; CYP72A154; 11-oxo-β-amyrin 30-monooxygenase
Systematic name: 11-oxo-β-amyrin,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (30-hydroxylating)
Comments: A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plant Glycyrrhiza uralensis (licorice) is involved in the biosynthesis of the triterpenoid saponin glycyrrhizin. The enzyme from the plant Medicago truncatula can also hydroxylate β-amyrin.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
1.  Seki, H., Sawai, S., Ohyama, K., Mizutani, M., Ohnishi, T., Sudo, H., Fukushima, E.O., Akashi, T., Aoki, T., Saito, K. and Muranaka, T. Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin. Plant Cell 23 (2011) 4112–4123. [DOI] [PMID: 22128119]
[EC created 2013 as EC, transferred 2018 to EC]
Accepted name: glycyrrhizin hydrolase
Reaction: glycyrrhizin + H2O = β-D-glucuronosyl-(1→2)-D-glucuronate + glycyrrhetinate
Glossary: glycyrrhizin = glycyrrhizinate
glycyrrhetinate = 3-β-glycyrrhetinate = 3β-hydroxy-11-oxoolean-12-en-30-oate
Other name(s): glycyrrhizinate β-glucuronidase; glycyrrhizin β-hydrolase; glycyrrhizinic acid hydrolase
Systematic name: glycyrrhizinate glucuronosylhydrolase
Comments: The enzyme from Aspergillus niger is specific for the hydrolysis of the triterpenoid glycoside glycyrrhizin from roots of Glycyrrhiza sp.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 102484-56-0
1.  Muro, T., Kuramoto, T., Imoto, K. and Okada, S. Purification and some properties of glycyrrhizinic acid hydrolase from Aspergillus niger GRM3. Agric. Biol. Chem. 50 (1986) 687–692.
[EC created 1989]

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