The Enzyme Database

Your query returned 4 entries.    printer_iconPrintable version



EC 1.1.1.230     
Accepted name: 3α-hydroxyglycyrrhetinate dehydrogenase
Reaction: 3α-hydroxyglycyrrhetinate + NADP+ = 3-oxoglycyrrhetinate + NADPH + H+
Systematic name: 3α-hydroxyglycyrrhetinate:NADP+ 3-oxidoreductase
Comments: Highly specific to 3α-hydroxy derivatives of glycyrrhetinate and its analogues. Not identical to EC 1.1.1.50 3α-hydroxysteroid dehydrogenase (Si-specific).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 114308-07-5
References:
1.  Akao, T., Akao, T., Hattori, M., Namba, T. and Kobashi, K. Purification and properties of 3α-hydroxyglycyrrhetinate dehydrogenase of Clostridium innocuum from human intestine. J. Biochem. (Tokyo) 103 (1988) 504–507. [PMID: 3164718]
[EC 1.1.1.230 created 1990]
 
 
EC 1.14.13.173      
Transferred entry: 11-oxo-β-amyrin 30-oxidase. Now EC 1.14.14.115, 11-oxo-β-amyrin 30-oxidase.
[EC 1.14.13.173 created 2013, deleted 2018]
 
 
EC 1.14.14.115     
Accepted name: 11-oxo-β-amyrin 30-oxidase
Reaction: 11-oxo-β-amyrin + 3 O2 + 3 [reduced NADPH—hemoprotein reductase] = glycyrrhetinate + 3 [oxidized NADPH—hemoprotein reductase] + 4 H2O (overall reaction)
(1a) 11-oxo-β-amyrin + O2 + [reduced NADPH—hemoprotein reductase] = 30-hydroxy-11-oxo-β-amyrin + [oxidized NADPH—hemoprotein reductase] + H2O
(1b) 30-hydroxy-11-oxo-β-amyrin + O2 + [reduced NADPH—hemoprotein reductase] = glycyrrhetaldehyde + [oxidized NADPH—hemoprotein reductase] + 2 H2O
(1c) glycyrrhetaldehyde + O2 + [reduced NADPH—hemoprotein reductase] = glycyrrhetinate + [oxidized NADPH—hemoprotein reductase] + H2O
For diagram of glycyrrhenate biosynthesis, click here
Other name(s): CYP72A; CYP72A154; 11-oxo-β-amyrin 30-monooxygenase
Systematic name: 11-oxo-β-amyrin,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (30-hydroxylating)
Comments: A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plant Glycyrrhiza uralensis (licorice) is involved in the biosynthesis of the triterpenoid saponin glycyrrhizin. The enzyme from the plant Medicago truncatula can also hydroxylate β-amyrin.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Seki, H., Sawai, S., Ohyama, K., Mizutani, M., Ohnishi, T., Sudo, H., Fukushima, E.O., Akashi, T., Aoki, T., Saito, K. and Muranaka, T. Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin. Plant Cell 23 (2011) 4112–4123. [DOI] [PMID: 22128119]
[EC 1.14.14.115 created 2013 as EC 1.14.13.173, transferred 2018 to EC 1.14.14.115]
 
 
EC 3.2.1.128     
Accepted name: glycyrrhizin hydrolase
Reaction: glycyrrhizin + H2O = β-D-glucuronosyl-(1→2)-D-glucuronate + glycyrrhetinate
Glossary: glycyrrhizin = glycyrrhizinate
glycyrrhetinate = 3-β-glycyrrhetinate = 3β-hydroxy-11-oxoolean-12-en-30-oate
Other name(s): glycyrrhizinate β-glucuronidase; glycyrrhizin β-hydrolase; glycyrrhizinic acid hydrolase
Systematic name: glycyrrhizinate glucuronosylhydrolase
Comments: The enzyme from Aspergillus niger is specific for the hydrolysis of the triterpenoid glycoside glycyrrhizin from roots of Glycyrrhiza sp.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 102484-56-0
References:
1.  Muro, T., Kuramoto, T., Imoto, K. and Okada, S. Purification and some properties of glycyrrhizinic acid hydrolase from Aspergillus niger GRM3. Agric. Biol. Chem. 50 (1986) 687–692.
[EC 3.2.1.128 created 1989]
 
 


Data © 2001–2021 IUBMB
Web site © 2005–2021 Andrew McDonald