| EC |
1.17.8.1 |
| Accepted name: |
hydroxysqualene dehydroxylase |
| Reaction: |
squalene + FAD + H2O = hydroxysqualene + FADH2 |
| Glossary: |
hydroxysqualene = (6E,10E,12R,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-12-ol |
| Other name(s): |
hpnE (gene name) |
| Systematic name: |
squalene:FAD oxidoreductase (hydroxylating) |
| Comments: |
This enzyme, isolated from the bacteria Rhodopseudomonas palustris and Zymomonas mobilis, participates, along with EC 2.5.1.103, presqualene diphosphate synthase, and EC 4.2.3.156, hydroxysqualene synthase, in the conversion of all-trans-farnesyl diphosphate to squalene. Eukaryotes achieve the same goal in a single step, catalysed by EC 2.5.1.21, squalene synthase. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Pan, J.J., Solbiati, J.O., Ramamoorthy, G., Hillerich, B.S., Seidel, R.D., Cronan, J.E., Almo, S.C. and Poulter, C.D. Biosynthesis of squalene from farnesyl diphosphate in bacteria: three steps catalyzed by three enzymes. ACS Cent. Sci. 1 (2015) 77–82. [DOI] [PMID: 26258173] |
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| [EC 1.17.8.1 created 2016] |
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| EC |
4.2.3.156 |
| Accepted name: |
hydroxysqualene synthase |
| Reaction: |
presqualene diphosphate + H2O = hydroxysqualene + diphosphate |
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For diagram of botryococcus braunii BOT22 squalene and botrycoccene biosynthesis, click here |
| Glossary: |
presqualene = {(1R,2R,3R)-2-[(3E)-4,8-dimethylnona-3,7-dien-1-yl]-2-methyl-3-[(2E,6E)-2,6,10-trimethylundeca-1,5,9-trien-1-yl]cyclopropan-1-yl}methyl
hydroxysqualene = (6E,10E,12R,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaen-12-ol |
| Other name(s): |
hpnC (gene name) |
| Systematic name: |
presqualene diphosphate diphosphate-lyase (adding water; hydroxyasqualene-forming) |
| Comments: |
This enzyme, isolated from the bacteria Rhodopseudomonas palustris and Zymomonas mobilis, participates, along with EC 2.5.1.103, presqualene diphosphate synthase, and EC 1.17.8.1, hydroxysqualene dehydroxylase, in the conversion of all-trans-farnesyl diphosphate to squalene. Eukaryotes achieve the same goal in a single step, catalysed by EC 2.5.1.21, squalene synthase. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
| References: |
| 1. |
Pan, J.J., Solbiati, J.O., Ramamoorthy, G., Hillerich, B.S., Seidel, R.D., Cronan, J.E., Almo, S.C. and Poulter, C.D. Biosynthesis of squalene from farnesyl diphosphate in bacteria: three steps catalyzed by three enzymes. ACS Cent. Sci. 1 (2015) 77–82. [DOI] [PMID: 26258173] |
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| [EC 4.2.3.156 created 2016] |
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