EC |
1.7.3.2 |
Accepted name: |
acetylindoxyl oxidase |
Reaction: |
N-acetylindoxyl + O2 = N-acetylisatin + (?) |
Systematic name: |
N-acetylindoxyl:oxygen oxidoreductase |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 9029-37-2 |
References: |
1. |
Beevers, H. and French, R.C. Oxidation of N-acetylindoxyl by an enzyme from plants. Arch. Biochem. Biophys. 50 (1954) 427–439. [DOI] [PMID: 13159343] |
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[EC 1.7.3.2 created 1961] |
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EC |
1.14.13.235 |
Accepted name: |
indole-3-acetate monooxygenase |
Reaction: |
(indol-3-yl)acetate + NADH + H+ + O2 = (2-hydroxy-1H-indol-3-yl)acetate + NAD+ + H2O |
Glossary: |
(indol-3-yl)acetate =(1H-indol-3-yl)acetate = indole-3-acetate |
Other name(s): |
iacA (gene name) |
Systematic name: |
(indol-3-yl)acetate,NADH:oxygen oxidoreductase (2-hydroxylating) |
Comments: |
The enzyme, characterized from Pseudomonas putida strains, catalyses the first step in a pathway for degradation of the plant hormone indole-3-acetate. When acting on indole, the enzyme forms indoxyl, which reacts spontaneously with oxygen to form the blue dye indigo. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Leveau, J.H. and Lindow, S.E. Utilization of the plant hormone indole-3-acetic acid for growth by Pseudomonas putida strain 1290. Appl. Environ. Microbiol. 71 (2005) 2365–2371. [DOI] [PMID: 15870323] |
2. |
Scott, J.C., Greenhut, I.V. and Leveau, J.H. Functional characterization of the bacterial iac genes for degradation of the plant hormone indole-3-acetic acid. J Chem Ecol 39 (2013) 942–951. [DOI] [PMID: 23881445] |
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[EC 1.14.13.235 created 2017] |
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EC |
2.4.1.220 |
Accepted name: |
indoxyl-UDPG glucosyltransferase |
Reaction: |
UDP-glucose + indoxyl = UDP + indican |
Glossary: |
indoxyl = indole-3-ol |
Other name(s): |
indoxyl-UDPG-glucosyltransferase |
Systematic name: |
UDP-glucose:indoxyl 3-O-β-D-glucosyltransferase |
Comments: |
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 258339-72-9 |
References: |
1. |
Marcinek, H., Weyler, W., Deus-Neumann, B. and Zenk, M.H. Indoxyl-UDPG-glucosyltransferase from Baphicacanthus cusia. Phytochemistry 53 (2000) 201–207. [DOI] [PMID: 10680172] |
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[EC 2.4.1.220 created 2002] |
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EC |
3.1.1.56 |
Accepted name: |
methylumbelliferyl-acetate deacetylase |
Reaction: |
4-methylumbelliferyl acetate + H2O = 4-methylumbelliferone + acetate |
Other name(s): |
esterase D |
Systematic name: |
4-methylumbelliferyl-acetate acylhydrolase |
Comments: |
Acts on short-chain acyl esters of 4-methylumbelliferone, but not on naphthyl, indoxyl or thiocholine esters. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 83380-83-0 |
References: |
1. |
Hopkinson, D.A., Mestriner, M.A., Cortner, J. and Harris, H. Esterase D: a new human polymorphism. Ann. Hum. Genet. 37 (1973) 119–137. [DOI] [PMID: 4768551] |
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[EC 3.1.1.56 created 1986] |
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