EC
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3.2.1.134
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Transferred entry: | difructose-dianhydride-I hydrolase. Now EC 4.2.1.179, difructose-dianhydride-I hydro-lyase
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[EC 3.2.1.134 created 1992, deleted 2021] |
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EC |
4.2.1.178 |
Accepted name: |
difructose-dianhydride-III synthase |
Reaction: |
inulobiose = α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride + H2O |
Glossary: |
difructose anhydride III = α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride
inulobiose = β-D-fructofuranosyl-(2→1)-D-fructose |
Other name(s): |
DFA-IIIase; difructose anhydride III hydrolase |
Systematic name: |
inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride-forming) |
Comments: |
The enzyme participates in an inulin degradation pathway, in which it forms inulobiose from difructose anhydride III. A conformational change in the enzyme from the bacterium Pseudarthrobacter chlorophenolicus results in it also catalysing the activity of EC 4.2.2.18, inulin fructotransferase (DFA-III-forming). |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc |
References: |
1. |
Tanaka, T., Uchiyama, T., Kobori, H. and Tanaka, K. Enzymic hydrolysis of di-D-fructofuranose 1, 2′; 2, 3′ dianhydride with Arthrobacter ureafaciens. J. Biochem. 78 (1975) 1201–1206. [DOI] [PMID: 1225919] |
2. |
Neubauer, A., Walter, M., and Buchholz, K. Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124. Biocatalysis and Biotransformation 18 (2000) 443–455. [DOI] |
3. |
Saito, K., Sumita, Y., Nagasaka, Y., Tomita, F. and Yokota, A. Molecular cloning of the gene encoding the di-D-fructofuranose 1,2′:2,3′ dianhydride hydrolysis enzyme (DFA IIIase) from Arthrobacter sp. H65-7. J. Biosci. Bioeng. 95 (2003) 538–540. [DOI] [PMID: 16233453] |
4. |
Yu, S., Wang, X., Zhang, T., Stressler, T., Fischer, L., Jiang, B. and Mu, W. Identification of a novel di-D-fructofuranose 1,2′:2,3′ dianhydride (DFA III) hydrolysis enzyme from Arthrobacter aurescens SK8.001. PLoS One 10:e0142640 (2015). [DOI] [PMID: 26555784] |
5. |
Yu, S., Shen, H., Cheng, Y., Zhu, Y., Li, X., and Mu, W. Structural and functional basis of difructose anhydride III hydrolase, which sequentially converts inulin using the same catalytic residue. ACS Catalysis 8 (2018) 10683–10697. [DOI] |
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[EC 4.2.1.178 created 2021] |
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EC |
4.2.1.179 |
Accepted name: |
difructose-anhydride-I synthase |
Reaction: |
inulobiose = bis-D-fructose 2′,1:2,1′-dianhydride + H2O |
Glossary: |
α-D-fructofuranose-β-D-fructofuranose 2′,1:2,1′-dianhydride = bis-D-fructose 2′,1:2,1′-dianhydride = difructose anhydride I = DFA-I |
Other name(s): |
DFAIase; inulobiose hydrolase; bis-D-fructose 2′,1:2,1′-dianhydride fructohydrolase |
Systematic name: |
inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2′,1:2,1′-dianhydride-forming) |
Comments: |
The enzyme, studied in the fungus Aspergillus fumigatus, may participate in an inulin degradation pathway in which it converts the product of EC 4.2.2.17, inulin fructotransferase (DFA-I-forming), to inulobiose, though in vitro activity was higher in the direction of DFA-I formation. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 121479-55-8 |
References: |
1. |
Matsuyama, T. and Tanaka, K. On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose. Agric. Biol. Chem. 53 (1989) 831–832. |
2. |
Matsuyama, T., Tanaka, K., Mashiko, M. and Kanamoto, M. Enzymic formation of di-D-fructose 1,2′; 2,1′ dianhydride from inulobiose by Aspergillus fumigatus. J. Biochem. (Tokyo) 92 (1982) 1325–1328. [PMID: 6757245] |
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[EC 4.2.1.179 created 1992 as EC 3.2.1.134, transferred to EC 4.2.1.179 2021] |
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