The Enzyme Database

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Transferred entry: difructose-dianhydride-I hydrolase. Now EC, difructose-dianhydride-I hydro-lyase
[EC created 1992, deleted 2021]
Accepted name: difructose-dianhydride-III synthase
Reaction: inulobiose = α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride + H2O
Glossary: difructose anhydride III = α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride
inulobiose = β-D-fructofuranosyl-(2→1)-D-fructose
Other name(s): DFA-IIIase; difructose anhydride III hydrolase
Systematic name: inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2′,1:2,3′-dianhydride-forming)
Comments: The enzyme participates in an inulin degradation pathway, in which it forms inulobiose from difructose anhydride III. A conformational change in the enzyme from the bacterium Pseudarthrobacter chlorophenolicus results in it also catalysing the activity of EC, inulin fructotransferase (DFA-III-forming).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
1.  Tanaka, T., Uchiyama, T., Kobori, H. and Tanaka, K. Enzymic hydrolysis of di-D-fructofuranose 1, 2′; 2, 3′ dianhydride with Arthrobacter ureafaciens. J. Biochem. 78 (1975) 1201–1206. [DOI] [PMID: 1225919]
2.  Neubauer, A., Walter, M., and Buchholz, K. Formation of inulobiose from difructoseanhydride III catalysed by a lysate from Arthrobacter ureafaciens ATCC 21124. Biocatalysis and Biotransformation 18 (2000) 443–455. [DOI]
3.  Saito, K., Sumita, Y., Nagasaka, Y., Tomita, F. and Yokota, A. Molecular cloning of the gene encoding the di-D-fructofuranose 1,2′:2,3′ dianhydride hydrolysis enzyme (DFA IIIase) from Arthrobacter sp. H65-7. J. Biosci. Bioeng. 95 (2003) 538–540. [DOI] [PMID: 16233453]
4.  Yu, S., Wang, X., Zhang, T., Stressler, T., Fischer, L., Jiang, B. and Mu, W. Identification of a novel di-D-fructofuranose 1,2′:2,3′ dianhydride (DFA III) hydrolysis enzyme from Arthrobacter aurescens SK8.001. PLoS One 10:e0142640 (2015). [DOI] [PMID: 26555784]
5.  Yu, S., Shen, H., Cheng, Y., Zhu, Y., Li, X., and Mu, W. Structural and functional basis of difructose anhydride III hydrolase, which sequentially converts inulin using the same catalytic residue. ACS Catalysis 8 (2018) 10683–10697. [DOI]
[EC created 2021]
Accepted name: difructose-anhydride-I synthase
Reaction: inulobiose = bis-D-fructose 2′,1:2,1′-dianhydride + H2O
Glossary: α-D-fructofuranose-β-D-fructofuranose 2′,1:2,1′-dianhydride = bis-D-fructose 2′,1:2,1′-dianhydride = difructose anhydride I = DFA-I
Other name(s): DFAIase; inulobiose hydrolase; bis-D-fructose 2′,1:2,1′-dianhydride fructohydrolase
Systematic name: inulobiose hydro-lyase (α-D-fructofuranose-β-D-fructofuranose 2′,1:2,1′-dianhydride-forming)
Comments: The enzyme, studied in the fungus Aspergillus fumigatus, may participate in an inulin degradation pathway in which it converts the product of EC, inulin fructotransferase (DFA-I-forming), to inulobiose, though in vitro activity was higher in the direction of DFA-I formation.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 121479-55-8
1.  Matsuyama, T. and Tanaka, K. On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobiose. Agric. Biol. Chem. 53 (1989) 831–832.
2.  Matsuyama, T., Tanaka, K., Mashiko, M. and Kanamoto, M. Enzymic formation of di-D-fructose 1,2′; 2,1′ dianhydride from inulobiose by Aspergillus fumigatus. J. Biochem. (Tokyo) 92 (1982) 1325–1328. [PMID: 6757245]
[EC created 1992 as EC, transferred to EC 2021]

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