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Your query returned 9 entries. Printable version
EC | 1.1.3.43 | ||||||
Accepted name: | paromamine 6′-oxidase | ||||||
Reaction: | paromamine + O2 = 6′-dehydroparomamine + H2O2 | ||||||
Other name(s): | btrQ (gene name); neoG (gene name); kanI (gene name); tacB (gene name); neoQ (obsolete gene name) | ||||||
Systematic name: | paromamine:oxygen 6′-oxidoreductase | ||||||
Comments: | Contains FAD. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Works in combination with EC 2.6.1.93, neamine transaminase, to replace the 6′-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces fradiae also catalyses EC 1.1.3.44, 6′′′-hydroxyneomycin C oxidase. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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EC | 2.3.1.81 | ||||||
Accepted name: | aminoglycoside 3-N-acetyltransferase | ||||||
Reaction: | acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N3-acetyl-2-deoxystreptamine antibiotic | ||||||
For diagram of neamine and ribostamycin biosynthesis, click here | |||||||
Glossary: | kanamycin | ||||||
Other name(s): | 3-aminoglycoside acetyltransferase; 3-N-aminoglycoside acetyltransferase; aminoglycoside N3-acetyltransferase; acetyl-CoA:2-deoxystreptamine-antibiotic N3′-acetyltransferase (incorrect); aminoglycoside N3′-acetyltransferase (incorrect) | ||||||
Systematic name: | acetyl-CoA:2-deoxystreptamine-antibiotic N3-acetyltransferase | ||||||
Comments: | Different from EC 2.3.1.60 gentamicin 3-N-acetyltransferase. A wide range of antibiotics containing the 2-deoxystreptamine ring can act as acceptors, including gentamicin, kanamycin, tobramycin, neomycin and apramycin. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 60120-42-5 | ||||||
References: |
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EC | 2.4.1.301 | ||||||
Accepted name: | 2′-deamino-2′-hydroxyneamine 1-α-D-kanosaminyltransferase | ||||||
Reaction: | (1) UDP-α-D-kanosamine + 2′-deamino-2′-hydroxyneamine = UDP + kanamycin A (2) UDP-α-D-kanosamine + neamine = UDP + kanamycin B (3) UDP-α-D-kanosamine + paromamine = UDP + kanamycin C (4) UDP-α-D-kanosamine + 2′-deamino-2′-hydroxyparomamine = UDP + kanamycin X |
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For diagram of kanamycin A biosynthesis, click here | |||||||
Glossary: | neamine = (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2,6-diamino-2,6-dideoxy-α-D-glucopyranoside paromamine = (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-α-D-glucopyranoside UDP-α-D-kanosamine = uridine 5′-[3-(3-amino-3-deoxy-α-D-glucopyranosyl) diphosphate] kanamycin A = (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-α-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-α-D-glucopyranoside kanamycin B = (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-α-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-α-D-glucopyranoside kanamycin C = (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-α-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2-amino-2-deoxy-α-D-glucopyranoside kanamycin X = (1S,2R,3R,4S,6R)-4,6-diamino-3-(α-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-α-D-glucopyranoside |
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Other name(s): | kanE (gene name); kanM2 (gene name) | ||||||
Systematic name: | UDP-α-D-kanosamine:2′-deamino-2′-hydroxyneamine 1-α-D-kanosaminyltransferase | ||||||
Comments: | Involved in the biosynthetic pathway of kanamycins. The enzyme characterized from the bacterium Streptomyces kanamyceticus can also accept UDP-α-D-glucose with lower efficiency [2]. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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EC | 2.4.2.49 | ||||||
Accepted name: | neamine phosphoribosyltransferase | ||||||
Reaction: | neamine + 5-phospho-α-D-ribose 1-diphosphate = 5′′-phosphoribostamycin + diphosphate | ||||||
For diagram of neamine and ribostamycin biosynthesis, click here | |||||||
Glossary: | neamine = (2R,3S,4R,5R,6R)-5-amino-2-(aminomethyl)-6-{[(1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl]oxy}oxane-3,4-diol ribostamycin = (2R,3S,4R,5R,6R)-5-amino-2-(aminomethyl)-6-{[(1R,2R,3S,4R,6S)-4,6-diamino-2-{[(2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]oxy}-3-hydroxycyclohexyl]oxy}oxane-3,4-diol |
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Other name(s): | btrL (gene name); neoM (gene name) | ||||||
Systematic name: | neamine:5-phospho-α-D-ribose 1-diphosphate phosphoribosyltransferase | ||||||
Comments: | Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including ribostamycin, neomycin and butirosin. The enzyme requires a divalent metal ion, optimally Mg2+, Ni2+ or Co2+. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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EC | 2.6.1.93 | ||||||
Accepted name: | neamine transaminase | ||||||
Reaction: | neamine + 2-oxoglutarate = 6′-dehydroparomamine + L-glutamate | ||||||
For diagram of neamine and ribostamycin biosynthesis, click here | |||||||
Other name(s): | glutamate—6′-dehydroparomamine aminotransferase; btrB (gene name); neoN (gene name); kacL (gene name) | ||||||
Systematic name: | neamine:2-oxoglutarate aminotransferase | ||||||
Comments: | The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin B, butirosin, neomycin and ribostamycin. Works in combination with EC 1.1.3.43, paromamine 6-oxidase, to replace the 6′-hydroxy group of paromamine with an amino group. The enzyme from the bacterium Streptomyces kanamyceticus can also catalyse EC 2.6.1.94, 2′-deamino-2′-hydroxyneamine transaminase, which leads to production of kanamycin A [3]. The enzyme from the bacterium Streptomyces fradiae can also catalyse EC 2.6.1.95, leading to production of neomycin C [2]. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||
References: |
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EC | 2.6.1.94 | ||||||
Accepted name: | 2′-deamino-2′-hydroxyneamine transaminase | ||||||
Reaction: | 2′-deamino-2′-hydroxyneamine + 2-oxoglutarate = 2′-deamino-2′-hydroxy-6′-dehydroparomamine + L-glutamate | ||||||
Other name(s): | kacL (gene name) | ||||||
Systematic name: | 2′-deamino-2′-hydroxyneamine:2-oxoglutarate aminotransferase | ||||||
Comments: | The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of kanamycin A and kanamycin D. The enzyme, characterized from the bacterium Streptomyces kanamyceticus, can also catalyse EC 2.6.1.93, neamine transaminase. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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EC | 2.6.1.95 | ||||||
Accepted name: | neomycin C transaminase | ||||||
Reaction: | neomycin C + 2-oxoglutarate = 6′′′-deamino-6′′′-oxoneomycin C + L-glutamate | ||||||
Other name(s): | neoN (gene name) | ||||||
Systematic name: | 2-oxoglutarate:neomycin C aminotransferase | ||||||
Comments: | The reaction occurs in vivo in the opposite direction. Involved in the biosynthetic pathway of aminoglycoside antibiotics of the neomycin family. Works in combination with EC 1.1.3.44, 6′′′-hydroxyneomycin C oxidase, to replace the 6′′′-hydroxy group of 6′′′-deamino-6′′′-hydroxyneomycin C with an amino group. The enzyme, characterized from the bacterium Streptomyces fradiae, can also catalyse EC 2.6.1.93, neamine transaminase. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB | ||||||
References: |
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EC | 2.7.1.95 | ||||||
Accepted name: | kanamycin kinase | ||||||
Reaction: | ATP + kanamycin = ADP + kanamycin 3′-phosphate | ||||||
Glossary: | kanamycin | ||||||
Other name(s): | neomycin-kanamycin phosphotransferase; | ||||||
Systematic name: | ATP:kanamycin 3′-O-phosphotransferase | ||||||
Comments: | Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 62213-36-9 | ||||||
References: |
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EC | 3.1.3.88 | ||||||
Accepted name: | 5′′-phosphoribostamycin phosphatase | ||||||
Reaction: | 5′′-phosphoribostamycin + H2O = ribostamycin + phosphate | ||||||
For diagram of neamine and ribostamycin biosynthesis, click here | |||||||
Other name(s): | btrP (gene name); neoI (gene name) | ||||||
Systematic name: | 5′′-phosphoribostamycin phosphohydrolase | ||||||
Comments: | Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including ribostamycin, neomycin and butirosin. No metal is required for activity. | ||||||
Links to other databases: | BRENDA, EXPASY, KEGG, MetaCyc | ||||||
References: |
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