EC |
1.1.1.317 |
Accepted name: |
perakine reductase |
Reaction: |
raucaffrinoline + NADP+ = perakine + NADPH + H+ |
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For diagram of peraksine biosynthesis, click here |
Glossary: |
raucaffrinoline = (17R,20α,21β)-1,2-didehydro-1-demethyl-19-hydroxy-21-methyl-18-norajmalan-17-yl acetate
perakine = raucaffrine = (17R,20α,21β)-1,2-didehydro-1-demethyl-17-(acetyloxy)-21-methyl-18-norajmalan-19-al |
Systematic name: |
raucaffrinoline:NADP+ oxidoreductase |
Comments: |
The biosynthesis of raucaffrinoline from perakine is a side route of the ajmaline biosynthesis pathway. The enzyme is a member of the aldo-keto reductase enzyme superfamily from higher plants. |
Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB |
References: |
1. |
Sun, L., Ruppert, M., Sheludko, Y., Warzecha, H., Zhao, Y. and Stockigt, J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol. Biol. 67 (2008) 455–467. [DOI] [PMID: 18409028] |
2. |
Rosenthal, C., Mueller, U., Panjikar, S., Sun, L., Ruppert, M., Zhao, Y. and Stockigt, J. Expression, purification, crystallization and preliminary X-ray analysis of perakine reductase, a new member of the aldo-keto reductase enzyme superfamily from higher plants. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62 (2006) 1286–1289. [DOI] [PMID: 17142919] |
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[EC 1.1.1.317 created 2011] |
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EC |
3.1.1.6 |
Accepted name: |
acetylesterase |
Reaction: |
an acetic ester + H2O = an alcohol + acetate |
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For diagram of peraksine biosynthesis, click here |
Other name(s): |
C-esterase (in animal tissues); acetic ester hydrolase; chloroesterase; p-nitrophenyl acetate esterase; Citrus acetylesterase |
Systematic name: |
acetic-ester acetylhydrolase |
Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9000-82-2 |
References: |
1. |
Aldridge, W.N. Serum esterases. I. Two types of esterase (A and B) hydrolysing p-nitrophenyl acetate, propionate and butyrate and a method for their determination. Biochem. J. 53 (1953) 110–117. [PMID: 13032041] |
2. |
Bergmann, F. and Rimon, S. Fractionation of C-esterase from the hog's kidney extract. Biochem. J. 77 (1960) 209–214. [PMID: 16748846] |
3. |
Jansen, E.F., Nutting, M.-D.F. and Balls, A.K. The reversible inhibition of acetylesterase by diisopropyl fluorophosphate and tetraethyl pyrophosphate. J. Biol. Chem. 175 (1948) 975–987. [PMID: 18880795] |
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[EC 3.1.1.6 created 1961] |
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