The Enzyme Database

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EC 2.4.1.375     
Accepted name: rhamnogalacturonan I galactosyltransferase
Reaction: Transfer of a β-galactosyl residue in a β-(1→4) linkage from UDP-α-D-galactose to rhamnosyl residues within the rhamnogalacturonan I backbone.
Glossary: rhamnogalacturonan I backbone = [(1→2)-α-L-rhamnosyl-(1→4)-α-D-galacturonosyl]n
Systematic name: UDP-α-D-galactose:[rhamnogalacturonan I]-α-L-rhamnosyl β-1,4-galactosyltransferase (configuration-inverting)
Comments: The enzyme, characterized from the plant Vigna angularis (azuki beans), participates in the biosynthesis of rhamnogalacturonan I, one of the components of pectin in plant cell wall. It does not require any metal ions, and prefers substrates with a degree of polymerization larger than 9.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Matsumoto, N., Takenaka, Y., Wachananawat, B., Kajiura, H., Imai, T. and Ishimizu, T. Rhamnogalacturonan I galactosyltransferase: Detection of enzyme activity and its hyperactivation. Plant Physiol. Biochem. 142 (2019) 173–178. [DOI] [PMID: 31299599]
[EC 2.4.1.375 created 2020]
 
 
EC 3.2.1.171     
Accepted name: rhamnogalacturonan hydrolase
Reaction: Endohydrolysis of α-D-GalA-(1→2)-α-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with β-D-GalA at the reducing end.
Other name(s): rhamnogalacturonase A; RGase A; RG-hydrolase
Systematic name: rhamnogalacturonan α-D-GalA-(1→2)-α-L-Rha hydrolase
Comments: The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Petersen, T.N., Kauppinen, S. and Larsen, S. The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix. Structure 5 (1997) 533–544. [DOI] [PMID: 9115442]
2.  Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus. J. Biol. Chem. 269 (1994) 29182–29189. [PMID: 7961884]
3.  Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase. Glycobiology 5 (1995) 783–789. [DOI] [PMID: 8720076]
4.  Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus. Acta Crystallogr. D Biol. Crystallogr. 53 (1997) 105–107. [DOI] [PMID: 15299976]
5.  Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus. Biochem. Biophys. Res. Commun. 242 (1998) 552–559. [DOI] [PMID: 9464254]
[EC 3.2.1.171 created 2011]
 
 


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