© IUBMB 2001
The mechanism below rationalizes the reaction mechanism, see:
1. Harris, J.M., Gonzalez-Bello, C., Kleanthous, C., Hawkins, A.R., Coggins, J.R. and Abell, C. Evidence from kinetic isotope studies for an enolate intermediate in the mechanism of type II dehydroquinases. Biochem. J. 319 (1996) 333-336. [PMID: 8912664]
2. Leech, A.P., James, R,. Coggins, J.R. and Kleanthous, C. Mutagenesis of active site residues in type I dehydroquinase from Escherichia coli. Stalled catalysis in a histidine to alanine mutant. J. Biol. Chem. 270 (1995) 25827-25836. [PMID: 7592767]
3. Gourley, D.G., Shrive, A.K., Polikarpov, I., Krell, T., Coggins, J.R., Hawkins, A.R., Isaacs, N.W. and Sawyer, L. The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction. Nature Struct. Biol. 6 (1999) 521-525. [PMID: 10360352]
© IUBMB 2001
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shikimate biosynthesis
EC 4.2.1.10 3-dehydroquinate dehydratase