Mechanism of EC
3-dehydroquinate dehydratase

The mechanism below rationalizes the reaction mechanism, see:

1. Harris, J.M., Gonzalez-Bello, C., Kleanthous, C., Hawkins, A.R., Coggins, J.R. and Abell, C. Evidence from kinetic isotope studies for an enolate intermediate in the mechanism of type II dehydroquinases. Biochem. J. 319 (1996) 333-336. [PMID: 8912664]

2. Leech, A.P., James, R,. Coggins, J.R. and Kleanthous, C. Mutagenesis of active site residues in type I dehydroquinase from Escherichia coli. Stalled catalysis in a histidine to alanine mutant. J. Biol. Chem. 270 (1995) 25827-25836. [PMID: 7592767]

3. Gourley, D.G., Shrive, A.K., Polikarpov, I., Krell, T., Coggins, J.R., Hawkins, A.R., Isaacs, N.W. and Sawyer, L. The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction. Nature Struct. Biol. 6 (1999) 521-525. [PMID: 10360352]

© IUBMB 2001

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EC 3-dehydroquinate dehydratase