The enzyme from Apium graveolens (celery) oxidizes alditols with a minimum requirement of 2R chirality at the carbon adjacent to the primary carbon undergoing the oxidation. The enzyme is specific for NAD+ and does not use NADP+.
Stoop, J.M.H. and Pharr, D.M. Partial purification and characterization of mannitol: mannose 1-oxidoreductase from celeriac (Apium graveolens var. rapaceum) roots. Arch. Biochem. Biophys.298 (1992) 612–619. [PMID: 1416989]
Stoop, J.M.H., Williamson, J.D., Conkling, M.A. and Pharr, D.M. Purification of NAD-dependent mannitol dehydrogenase from celery suspension cultures. Plant Physiol. (1995)108 (1995) 1219–1225. [PMID: 7630943]
Williamson, J.D., Stoop, J.M.H., Massel, M.O., Conkling, M.A. and Pharr, D.M. Sequence analysis of a mannitol dehydrogenase cDNA from plants reveals a function for the pathogenesis-related protein ELI3. Proc. Natl. Acad. Sci. USA92 (1995) 7148–7152. [PMID: 7638158]
Stoop, J.M.H., Chilton, W.S. and Pharr, D.M. Substrate specificity of the NAD-dependent mannitol dehydrogenase from celery. Phytochemistry43 (1996) 1145–1150.