This bacterial enzyme is involved, along with EC 126.96.36.199, 3α-hydroxycholanate dehydrogenase (NAD+), or EC 188.8.131.522, 3α-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3β-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 184.108.40.2063, 3β-hydroxycholanate 3-dehydrogenase (NADP+).
Edenharder, R., Pfutzner, A. and Hammann, R. Characterization of NAD-dependent 3 α- and 3 β-hydroxysteroid dehydrogenase and of NADP-dependent 7 β-hydroxysteroid dehydrogenase from Peptostreptococcus productus. Biochim. Biophys. Acta1004 (1989) 230–238. [PMID: 2752021]
Edenharder, R. and Pfutzner, M. Partial purification and characterization of an NAD-dependent 3 β-hydroxysteroid dehydrogenase from Clostridium innocuum. Appl. Environ. Microbiol.55 (1989) 1656–1659. [PMID: 2764572]
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