This bacterial enzyme is involved, along with EC 184.108.40.206, 3α-hydroxycholanate dehydrogenase (NAD+), or EC 220.127.116.112, 3α-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3β-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 18.104.22.1683, 3β-hydroxycholanate 3-dehydrogenase (NADP+).
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Edenharder, R. and Pfutzner, M. Partial purification and characterization of an NAD-dependent 3 β-hydroxysteroid dehydrogenase from Clostridium innocuum. Appl. Environ. Microbiol.55 (1989) 1656–1659. [PMID: 2764572]
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