This bacterial enzyme is involved, along with EC 22.214.171.124, 3α-hydroxycholanate dehydrogenase (NAD+), or EC 126.96.36.1992, 3α-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3β-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 188.8.131.523, 3β-hydroxycholanate 3-dehydrogenase (NADP+).
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